Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Signal Transduction Protein Trafficking Vesicle Transport Regulation

Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)

Price and availability

351 792 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2320Y] to GCN2 (phospho T899)
  • Suitable for: Dot blot, WB
  • Reacts with: Human

You may also be interested in

Product image
Anti-ARF6 antibody (ab226389)
Product image
Anti-TMUB1 antibody [EPR14066] (ab180586)
Product image
Anti-IMPDH1 antibody (ab228002)
Product image
Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)

Overview

  • Product name

    Anti-GCN2 (phospho T899) antibody [EPR2320Y]
    See all GCN2 primary antibodies
  • Description

    Rabbit monoclonal [EPR2320Y] to GCN2 (phospho T899)
  • Host species

    Rabbit
  • Specificity

    GCN2 (phospho T899) antibody (ab75836) detects GCN2 phosphorylated at threonine 899.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cell lysates treated with Calyculin. Dot Blot: GCN2 (phospho T899) phospho peptide.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2320Y
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Images

  • Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)
    Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)
    All lanes : Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) grown in serum free media for 24hours, whole cell lysate
    Lane 2 : HeLa grown in serum free media for 24hours, then treated with 100nM Calyculin A for 30min, whole cell lysate
    Lane 3 : HeLa grown in serum free media for 24hours, then treated with 100nM Calyculin A for 30min, whole cell lysate. Then the membrane was incubated with alkaline phosphatase

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 187 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)
    Dot Blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)

    Dot blot analysis of GCN2 (phospho T899) phospho peptide (Lane 1) and GCN2 non-phospho peptide (Lane 2) labelling GCN2 (phospho T899) phospho peptide with ab75836 at a dilution of 1:1000 dilution (0.277μg/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1:20,000 dilution. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST .

  • Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)
    Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)
    All lanes : Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) at 1/1000 dilution (purified)

    Lane 1 : HeLa cell lysate - untreated
    Lane 2 : HeLa cell lysate - treated with Calyculin

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 187 kDa
    Observed band size: 220 kDa
    why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)
    Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)
    All lanes : Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) at 1/500 dilution (unpurified)

    Lane 1 : HeLa cell lysate - untreated
    Lane 2 : HeLa cell lysate - treated with Calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 187 kDa
    Observed band size: 220 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)
    Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836)

  •  
  • Product image

    Anti-GCN2 antibody [EPR5970(2)] (ab134053)

    Applications: Flow Cyt, ICC/IF, IHC-P, WB

  •  
  • Product image

    Anti-GCN2 antibody [EPR5970(2)] - BSA and Azide free (ab157775)

    Applications: Flow Cyt, ICC/IF, IHC-P, WB

  •  
  • Product image

    Anti-GCN2 (phospho T899) antibody [EPR2320Y] - BSA and Azide free (ab157776)

    Applications: Dot, WB

  •  
  • Product image

    Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427)

    Applications: IP, WB

Clear all

Recently viewed products

  •  
  • Product image

    Anti-ARID1A antibody [EPR13501] (ab182560)

  •  
  • Product image

    Anti-Ctip2 antibody [EPR23120-25] (ab240636)

  •  
  • Product image

    Anti-RHEB antibody [EPR2971] (ab92313)

  •  
  • Product image

    Anti-S100A9 antibody [EPR22332-75] (ab242945)

  •  
  • Product image

    Anti-Glutamine Synthetase antibody (ab64613)

  •  
  • Product image

    Anti-TCEA1 antibody [EPR14821] (ab185947)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.