Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Phospholamban antibody [EPR21897] (ab219626)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21897] to Phospholamban
Suitable for: WB, IHC-P, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Phospholamban antibody [EPR21897]
See all Phospholamban primary antibodies
Description

Rabbit monoclonal [EPR21897] to Phospholamban
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human heart and fetal heart tissue lysates; Mouse heart tissue lysate; Rat heart tissue lysate. IHC-P: Human, mouse and rat cardiac muscle tissue. IP: Human fetal heart tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21897
Isotype

IgG
Research areas

Western blot - Anti-Phospholamban antibody [EPR21897] (ab219626)

All lanes : Anti-Phospholamban antibody [EPR21897] (ab219626) at 1/2000 dilution

Lane 1 : Human heart tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Mouse heart tissue lysate
Lanes 4-5 : Rat heart tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 6 kDa
Observed band size: 10,25 kDa
why is the actual band size different from the predicted?

Exposure time: 15 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

WB bands are consistent with reference (PMID 20696159; PMID 22144574)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (ab219626)

Immunohistochemical analysis of paraffin embedded rat cardiac muscle tissue labeling Phospholamban with ab219626 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human cardiac muscle (PMID: 28759816, PMID: 27642167) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (ab219626)

Negative control: No staining shown in human tonsil tissue.

Immunohistochemical analysis of paraffin embedded human tonsil tissue labeling Phospholamban with ab219626 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. No staining on human tonsil is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (ab219626)

Immunohistochemical analysis of paraffin embedded mouse cardiac muscle tissue labeling Phospholamban with ab219626 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on mouse cardiac muscle (PMID: 28759816, PMID: 27642167) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunoprecipitation - Anti-Phospholamban antibody [EPR21897] (ab219626)

Phospholamban was immunoprecipitated from 0.35 mg of human fetal heart tissue lysate with ab219626 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219626 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: Human fetal heart tissue lysate 10 μg (Input).
Lane 2: ab219626 IP in human fetal heart tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219626 in human fetal heart tissue lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (ab219626)

Immunohistochemical analysis of paraffin embedded human cardiac muscle tissue labeling Phospholamban with ab219626 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human cardiac muscle (PMID: 28759816, PMID: 27642167) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-Phospholamban antibody [EPR21897] (ab219626)

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