Anti-Solo antibody [EPR13772-74] (ab192873)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13772-74] to Solo
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Solo antibody [EPR13772-74]
See all Solo primary antibodies -
Description
Rabbit monoclonal [EPR13772-74] to Solo -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseWB MouseHuman -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain tissue lysate; HepG2 and Raw264.7 cell lysates; mouse brain, mouse kidney and rat brain tissue lysates; Neuro-2a cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13772-74 -
Isotype
IgG -
Research areas
Images
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Immunofluorescence analysis of Neuro2a cells labeling Solo using ab192873 at 1/50 dilution (20 μg/ml). A Goat anti rabbit IgG (Alexa Fluor®488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permealized with 0.1% triton X-100. Counterstain: DAPI.
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All lanes : Anti-Solo antibody [EPR13772-74] (ab192873) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Raw264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDa
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All lanes : Anti-Solo antibody [EPR13772-74] (ab192873) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDa
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