Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-Aldolase antibody [EPR23181-39]
See all Aldolase primary antibodies
Description

Rabbit monoclonal [EPR23181-39] to Aldolase
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse Human
ICC	Mouse Human
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MCF-7, HepG2, SH-SY5Y,THP-1, C2C12, C6, A549 and NIH/3T3 whole cell lysates; human brain, colon, colon cancer and skeletal muscle tissue lysates; Mouse brain and skeletal muscle tissue lysates; rat brain and skeletal muscle tissue lysates. IHC-P: Human lung cancer and colon cancer tissue; Mouse skeletal muscle tissue; Rat skeletal muscle tissue. ICC: A549 and NIH/3T3 cells. Flow cyt: A549 and NIH/3T3 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23181-39
Isotype

IgG
Research areas

Immunocytochemistry - Anti-Aldolase antibody [EPR23181-39] (ab252953)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A549 cells labelling Aldolase with ab252953 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing strong cytoplasmic and weak nuclear staining in A549 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/ml) dilution.
Western blot - Anti-Aldolase antibody [EPR23181-39] (ab252953)

All lanes : Anti-Aldolase antibody [EPR23181-39] (ab252953) at 1/1000 dilution

Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 40 µg
Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 40 µg
Lane 3 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 40 µg
Lane 4 : THP-1 (human monocytic leukemia monocyte) whole cell lysate at 40 µg
Lane 5 : C2C12 (mouse myoblasts myoblast) whole cell lysate at 40 µg
Lane 6 : C6 (rat glial tumor glial cell) whole cell lysate at 40 µg
Lane 7 : A549 (human lu carcinoma epithelial cell) whole cell lysate
Lane 8 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 40 µg

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

Predicted band size: 39 kDa
Observed band size: 39 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure times: Lanes 1-6: 5.5 seconds; Lanes 7-8: 26 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase antibody [EPR23181-39] (ab252953)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Aldolase with ab252953 at 1/50 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in rat skeletal muscle (PMID: 18676612). The section was incubated with ab252953 for 20 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Flow Cytometry - Anti-Aldolase antibody [EPR23181-39] (ab252953)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized A549 (Human lung carcinoma epithelial cell) cells labelling Aldolase with ab252953 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-Aldolase antibody [EPR23181-39] (ab252953)

All lanes : Anti-Aldolase antibody [EPR23181-39] (ab252953) at 1/1000 dilution

Lane 1 : Rat brain tissue lysate
Lane 2 : Rat skeletal muscle tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 39 kDa
Observed band size: 39 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure times: Lane 1: 10 seconds; Lane 2: 3 seconds.
Flow Cytometry - Anti-Aldolase antibody [EPR23181-39] (ab252953)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling Aldolase with ab252953 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunocytochemistry - Anti-Aldolase antibody [EPR23181-39] (ab252953)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Aldolase with ab252953 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing strong cytoplasmic and weak nuclear staining in NIH/3T3 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase antibody [EPR23181-39] (ab252953)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Aldolase with ab252953 at 1/50 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse skeletal muscle (PMID: 18676612). The section was incubated with ab252953 for 20 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-Aldolase antibody [EPR23181-39] (ab252953)

All lanes : Anti-Aldolase antibody [EPR23181-39] (ab252953) at 1/1000 dilution

Lane 1 : Human brain tissue lysate
Lane 2 : Human skeletal muscle tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Mouse skeletal muscle tissue lysate
Lane 5 : Human colon cancer tissue lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

Predicted band size: 39 kDa
Observed band size: 39 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure times: Lanes 1-3: 3 seconds; Lane 4-5: 5.5 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase antibody [EPR23181-39] (ab252953)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling Aldolase with ab252953 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human colon cancer (PMID: 29453983). The section was incubated with ab252953 for 20 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase antibody [EPR23181-39] (ab252953)

Immunohistochemical analysis of paraffin-embedded Human lung cancer (A) and its adjacent noncancerous tissue (B) tissue labeling Aldolase with ab252953 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human lung cancer (A) while weak or negative staining in its adjacent noncancerous tissue (B) (PMID: 24465716). The section was incubated with ab252953 for 20 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Anti-Aldolase antibody [EPR23181-39] (ab252953)