Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (ab250666)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14925] to SUCLA2 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, ICC, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free
    See all SUCLA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14925] to SUCLA2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, ICC, IP, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250666 is the carrier-free version of ab183513.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (ab250666)
    Western blot - Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (ab250666)
    All lanes : Anti-SUCLA2 antibody [EPR14925] (ab183513) at 1/50000 dilution

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Molt-4 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa



    This data was developed using ab183513, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (ab250666)
    Immunoprecipitation - Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (ab250666)
    This data was developed using ab183513, the same antibody clone in a different buffer formulation.Western blot analysis of HepG2 cell lysate immunoprecipitated using ab183513 at 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG secondary antibody used at 1/1500 dilution.
  • Flow Cytometry - Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (ab250666)
    Flow Cytometry - Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (ab250666)
    This data was developed using ab183513, the same antibody clone in a different buffer formulation.Flow cytometry analysis of 2% paraformaldehyde fixed 293 cells labeling SUCLA2 using ab183513 at a 1/100 dilution (green) or Rabbit monoclonal IgG (red), using a Goat anti rabbit IgG (FITC) secondary antibody at a 1/150 dilution.
  • Immunocytochemistry - Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (ab250666)
    Immunocytochemistry - Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (ab250666)
    This data was developed using ab183513, the same antibody clone in a different buffer formulation.Immunofluorescent staining of 4% paraformaldehyde fixed 293 cells, labeling SUCLA2 using ab183513 at a 1/250 dilution and a Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at a 1/200 dilution and counterstained with Dapi.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (ab250666)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (ab250666)
    This data was developed using ab183513, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling SUCLA2 using ab183513 at a 1/250 dilution, using a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (ab250666)
    Anti-SUCLA2 antibody [EPR14925] - BSA and Azide free (ab250666)

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