Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (ab255766)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [PMc 1D1] to CNGA1 - BSA and Azide free
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free
    See all CNGA1 primary antibodies

  • Description

    Mouse monoclonal [PMc 1D1] to CNGA1 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Rat and mouse retina tissue. IHC-Fr: Rat and mouse retina tissue.

  • General notes

    ab255766 is the carrier-free version of ab253296.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (ab255766)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (ab255766)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (ab253296).

    Immunohistochemical analysis of paraffin-embedded rat retina tissue labeling CNGA1 with ab253296 at 1.01µg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on light-sensing cells of rat retina is observed. The section was incubated with ab253296 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (ab255766)
    Immunohistochemistry (Frozen sections) - Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (ab255766)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (ab253296).

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat retina tissue labeling CNGA1 with ab253296 at 1/100 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at a 1/1000 dilution. Positive staining on rat retina is observed. Nuclear counterstain is DAPI.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at a 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (ab255766)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (ab255766)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (ab253296).

    Immunohistochemical analysis of paraffin-embedded mouse retina tissue labeling CNGA1 with ab253296 at 1.01µg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on light-sensing cells of mouse retina is observed. The section was incubated with ab253296 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (ab255766)
    Immunohistochemistry (Frozen sections) - Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (ab255766)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (ab253296).

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse retina tissue labeling CNGA1 with ab253296 at 5.05µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at a 1/1000 dilution. Positive staining on mouse retina is observed. Nuclear counterstain is DAPI.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at a 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (ab255766)
    Anti-CNGA1 antibody [PMc 1D1] - BSA and Azide free (ab255766)

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