Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 09, 2021

Anti-PGAM2 antibody [EPR15194(B)] (ab187147)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15194(B)] to PGAM2
Suitable for: WB, IHC-P, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-PGAM2 antibody [EPR15194(B)]
See all PGAM2 primary antibodies
Description

Rabbit monoclonal [EPR15194(B)] to PGAM2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human skeletal muscle tissue; Human, Rat and Mouse skeletal muscle tissue lysates, Human fetal heart tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15194(B)
Isotype

IgG

Western blot - Anti-PGAM2 antibody [EPR15194(B)] (ab187147)

All lanes : Anti-PGAM2 antibody [EPR15194(B)] (ab187147) at 1/10000 dilution

Lane 1 : Human skeletal muscle tissue lysate
Lane 2 : Human fetal heart tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 29 kDa
Observed band size: 25 kDa
why is the actual band size different from the predicted?
Western blot - Anti-PGAM2 antibody [EPR15194(B)] (ab187147)

All lanes : Anti-PGAM2 antibody [EPR15194(B)] (ab187147) at 1/10000 dilution

Lane 1 : Rat skeletal muscle tissue lysate
Lane 2 : Mouse skeletal muscle tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 29 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM2 antibody [EPR15194(B)] (ab187147)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue, labeling PGAM2 with ab187147 at 1/4000 dilution. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-PGAM2 antibody [EPR15194(B)] (ab187147)

Western blot analysis of PGAM2 in immunoprecipitation pellets from Human skeletal muscle lysate. ab187147 used at 1/50 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1000 dilution.
Anti-PGAM2 antibody [EPR15194(B)] (ab187147)

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