Anti-PGAM2 antibody [EPR15193(B)] (ab183027)
![Anti-PGAM2 antibody [EPR15193(B)] (ab183027)](https://www.abcam.com/ps/products/183/ab183027/Images/ab183027-215691-anti-pgam2-antibody-epr15193b-western-blot.jpg "Anti-PGAM2 antibody [EPR15193(B)] (ab183027)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15193(B)] to PGAM2
- Suitable for: IP, IHC-P, Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-PGAM2 antibody [EPR15193(B)]
See all PGAM2 primary antibodies -
Description
Rabbit monoclonal [EPR15193(B)] to PGAM2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanIP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human skeletal muscle lysate; Human fetal heart lysate; Human skeletal muscle tissue; Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR15193(B) -
Isotype
IgG
Images
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Western blot - Anti-PGAM2 antibody [EPR15193(B)] (ab183027)All lanes : Anti-PGAM2 antibody [EPR15193(B)] (ab183027) at 1/20000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 29 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM2 antibody [EPR15193(B)] (ab183027)](https://www.abcam.com/ps/products/183/ab183027/Images/ab183027-215689-ab183027IHC.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM2 antibody [EPR15193(B)] (ab183027)Immunohistochemistry analysis of paraffin embedded Human skeletal muscle tissue, labeling PGAM2 with ab183027 at 1/500 dilution. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-PGAM2 antibody [EPR15193(B)] (ab183027)](https://www.abcam.com/ps/products/183/ab183027/Images/ab183027-215690-anti-pgam2-antibody-epr15193b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PGAM2 antibody [EPR15193(B)] (ab183027)Immunopreciptation analysis of Human skletal muscle lysate, labeling PGAM2 with ab183027 at 1/50 dilution (lane 1) and negative control (lane 2) Anti-Rabbit IgG (HRP) secondary antibody used at 1/1500 dilution (lane 1) and blocking buffer was 5% NFDM/TBST.
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![Flow Cytometry - Anti-PGAM2 antibody [EPR15193(B)] (ab183027)](https://www.abcam.com/ps/products/183/ab183027/Images/ab183027-215688-anti-pgam2-antibody-epr15193b-flow-cytometry.jpg)
Flow Cytometry - Anti-PGAM2 antibody [EPR15193(B)] (ab183027)Flow cytometry analysis of Jurkat cells fixed with 2% paraformaldehyde, labeling PGAM2 with ab183027 at 1/20 dilution. Goat
anti Rabbit IgG secondary antibody used at 1/150 dilution. -
![Anti-PGAM2 antibody [EPR15193(B)] (ab183027)](https://www.abcam.com/ps/products/183/ab183027/Images/ab183027-5-benefits-of-recombinant-antibodies.png)
Anti-PGAM2 antibody [EPR15193(B)] (ab183027)
