Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PGAM2 antibody [EPR15193(B)] (ab183027)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15193(B)] to PGAM2
Suitable for: IP, IHC-P, Flow Cyt, WB
Reacts with: Human

Product name

Anti-PGAM2 antibody [EPR15193(B)]
See all PGAM2 primary antibodies
Description

Rabbit monoclonal [EPR15193(B)] to PGAM2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human skeletal muscle lysate; Human fetal heart lysate; Human skeletal muscle tissue; Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR15193(B)
Isotype

IgG

Western blot - Anti-PGAM2 antibody [EPR15193(B)] (ab183027)

All lanes : Anti-PGAM2 antibody [EPR15193(B)] (ab183027) at 1/20000 dilution

Lane 1 : Human skeletal muscle lysate
Lane 2 : Human fetal heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 29 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM2 antibody [EPR15193(B)] (ab183027)

Immunohistochemistry analysis of paraffin embedded Human skeletal muscle tissue, labeling PGAM2 with ab183027 at 1/500 dilution. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-PGAM2 antibody [EPR15193(B)] (ab183027)

Immunopreciptation analysis of Human skletal muscle lysate, labeling PGAM2 with ab183027 at 1/50 dilution (lane 1) and negative control (lane 2) Anti-Rabbit IgG (HRP) secondary antibody used at 1/1500 dilution (lane 1) and blocking buffer was 5% NFDM/TBST.
Flow Cytometry - Anti-PGAM2 antibody [EPR15193(B)] (ab183027)

Flow cytometry analysis of Jurkat cells fixed with 2% paraformaldehyde, labeling PGAM2 with ab183027 at 1/20 dilution. Goat
anti Rabbit IgG secondary antibody used at 1/150 dilution.
Anti-PGAM2 antibody [EPR15193(B)] (ab183027)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com