Anti-Paxillin antibody (ab264125)
Key features and details
- Rabbit polyclonal to Paxillin
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Paxillin antibody
See all Paxillin primary antibodies -
Description
Rabbit polyclonal to Paxillin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
-
Immunogen
Synthetic peptide within Human Paxillin aa 1-50. The exact sequence is proprietary.
Database link: P49023 -
Positive control
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human non-small cell lung cancer tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Paxillin antibody (ab264125)All lanes : Anti-Paxillin antibody (ab264125) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Predicted band size: 65 kDa
Exposure time: 10 seconds4-20% SDS-PAGE.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Paxillin antibody (ab264125)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human non-small cell lung cancer tissue labeling Paxillin with ab264125 at 1/200 dilution. Detection: DAB.
-
Immunoprecipitation - Anti-Paxillin antibody (ab264125)Paxillin was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264125 at 3ug/mg lysate. Western blot was performed from the immunoprecipitate using ab264125 at 0.04ug/ml.
Lane 1: HeLa whole cell lysate 50ug (Input).
Lane 2: ab264125 IP in HeLa whole cell lysate.
Lane 3: Control IgG.Exposure time: 10 secs.
