Anti-Cathepsin D antibody (ab217310)
Key features and details
- Rabbit polyclonal to Cathepsin D
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Cathepsin D antibody
See all Cathepsin D primary antibodies -
Description
Rabbit polyclonal to Cathepsin D -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human Cathepsin D aa 100-200 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P07339 -
Positive control
- Rat kidney and Human colon carcinoma lysates. U138 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin D antibody (ab217310)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human Skin with ab217310 at 1/200
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Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin D antibody (ab217310)Immunofluorescence analysis of formalin-fixed U138 cells, labeling Cathepsin D using ab217310 at a 1/50 dilution; followed by goat anti-rabbit lgG (H+L) Alexa Fluor 488-conjugated secondary antibody.
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Western blot - Anti-Cathepsin D antibody (ab217310)All lanes : Anti-Cathepsin D antibody (ab217310) at 1/200 dilution
Lane 1 : rat kidney lysates
Lane 2 : Human colon carcinoma lysates
Secondary
All lanes : goat anti-rabbit antibody (H+L) HRP-conjugated at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
