Anti-Pan3 antibody (ab88642)
Key features and details
- Rabbit polyclonal to Pan3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Pan3 antibody -
Description
Rabbit polyclonal to Pan3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Pan3 aa 400-500 conjugated to keyhole limpet haemocyanin.
(Peptide available asab102602) -
Positive control
- This antibody gave a positive signal in the following lysates: HeLa Whole Cell; HepG2 Whole Cell; MOLT4 Whole Cell; Jurkat Whole Cell; Human Liver Tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatApplication Abreviews Notes WB (2) Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 82 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 82 kDa).Target
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Function
Functions in cytoplasmic mRNA decay. As part of the Pan nuclease complex, recruits polyadenylate-binding protein which in turn stimulates PAN2 nuclease activity. -
Sequence similarities
Belongs to the protein kinase superfamily.
Contains 1 protein kinase domain. -
Domain
The protein kinase domain is predicted to be catalytically inactive. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 255967 Human
- Entrez Gene: 72587 Mouse
- Entrez Gene: 360760 Rat
- SwissProt: Q58A45 Human
- SwissProt: Q640Q5 Mouse
- Unigene: 645015 Human
- Unigene: 320469 Mouse
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Alternative names
- hPan3 antibody
- PAB dependent poly(A) specific ribonuclease subunit 3 antibody
- PAB-dependent poly(A)-specific ribonuclease subunit 3 antibody
see all
Images
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Western blot - Anti-Pan3 antibody (ab88642)All lanes : Anti-Pan3 antibody (ab88642) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 5 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 82 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 160 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Datasheets and documents
References (1)
ab88642 has been referenced in 1 publication.
- Yang S et al. USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization. Nat Commun 9:1285 (2018). PubMed: 29599486
Images
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Western blot - Anti-Pan3 antibody (ab88642)All lanes : Anti-Pan3 antibody (ab88642) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 5 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 82 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 160 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute