Antibodies, Proteins, Kits and Reagents for Life Science

281 433 ₸ Product size

100 µl 281 433 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Pan Trk antibody [EPR18413] (ab189903)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18413] to Pan Trk
Suitable for: IP, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Pan Trk antibody [EPR18413]
See all Pan Trk primary antibodies
Description

Rabbit monoclonal [EPR18413] to Pan Trk
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Mouse

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human, mouse and rat brain lysates; SH-SY5Y whole cell lysate; HEK-293 whole cell lysate transfected with TrkA, TrkB and TrkC, respectively. IP: Mouse brain whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Mouse
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18413
Isotype

IgG
Research areas

Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903)

All lanes : Anti-Pan Trk antibody [EPR18413] (ab189903) at 1/1000 dilution

Lane 1 : Human brain lysate
Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 94 kDa
Observed band size: 140,30 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

TrkB is abundantly expressed in the central and peripheral nervous system. The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.
Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903)

All lanes : Anti-Pan Trk antibody [EPR18413] (ab189903) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat heart lysate
Lane 7 : Rat kidney lysate
Lane 8 : Rat spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 94 kDa
Observed band size: 140,30 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

TrkB is abundantly expressed in the central and peripheral nervous system. The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.
Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903)

All lanes : Anti-Pan Trk antibody [EPR18413] (ab189903) at 1/5000 dilution

Lanes 1 & 3 & 5 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with empty vector (vector control)
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkA with His/Myc-tag
Lane 4 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB with His/Myc-tag
Lane 6 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkC with His/Myc-tag

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 94 kDa

Exposure times: Lane 1-2: 1 minute; Lane 3-6: 30 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-Pan Trk antibody [EPR18413] (ab189903)

TrkC (phospho Y820) was immunoprecipitated from 1 mg of mouse brain whole cell lysate with ab189903 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab189903 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.

Lane 1: Mouse brain whole cell lysate 10 µg (Input).

Lane 2: ab189903 IP in mouse brain whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab189903 in mouse brain whole cell lysate.

Exposure time: 5 seconds.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-Pan Trk antibody [EPR18413] (ab189903)

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