Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Pan Trk antibody [EPR18413] (ab189903)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18413] to Pan Trk
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Pan Trk antibody [EPR18413]
    See all Pan Trk primary antibodies

  • Description

    Rabbit monoclonal [EPR18413] to Pan Trk

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human, mouse and rat brain lysates; SH-SY5Y whole cell lysate; HEK-293 whole cell lysate transfected with TrkA, TrkB and TrkC, respectively. IP: Mouse brain whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903)
    Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903)
    All lanes : Anti-Pan Trk antibody [EPR18413] (ab189903) at 1/1000 dilution

    Lane 1 : Human brain lysate
    Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
    Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 94 kDa
    Observed band size: 140,30 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    TrkB is abundantly expressed in the central and peripheral nervous system. The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.

  • Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903)
    Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903)
    All lanes : Anti-Pan Trk antibody [EPR18413] (ab189903) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Rat heart lysate
    Lane 7 : Rat kidney lysate
    Lane 8 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 94 kDa
    Observed band size: 140,30 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    TrkB is abundantly expressed in the central and peripheral nervous system. The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.

  • Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903)
    Western blot - Anti-Pan Trk antibody [EPR18413] (ab189903)
    All lanes : Anti-Pan Trk antibody [EPR18413] (ab189903) at 1/5000 dilution

    Lanes 1 & 3 & 5 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with empty vector (vector control)
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkA with His/Myc-tag
    Lane 4 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB with His/Myc-tag
    Lane 6 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkC with His/Myc-tag

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 94 kDa



     

    Exposure times: Lane 1-2: 1 minute; Lane 3-6: 30 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Immunoprecipitation - Anti-Pan Trk antibody [EPR18413] (ab189903)
    Immunoprecipitation - Anti-Pan Trk antibody [EPR18413] (ab189903)

    TrkC (phospho Y820) was immunoprecipitated from 1 mg of mouse brain whole cell lysate with ab189903 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab189903 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.

    Lane 1: Mouse brain whole cell lysate 10 µg (Input).

    Lane 2: ab189903 IP in mouse brain whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab189903 in mouse brain whole cell lysate.

    Exposure time: 5 seconds.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

     

  • Anti-Pan Trk antibody [EPR18413] (ab189903)
    Anti-Pan Trk antibody [EPR18413] (ab189903)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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