Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Neurturin antibody [EPR23301-10] - BSA and Azide free (ab274426)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23301-10] to Neurturin - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Neurturin antibody [EPR23301-10] - BSA and Azide free
    See all Neurturin primary antibodies

  • Description

    Rabbit monoclonal [EPR23301-10] to Neurturin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human heart, testis and brain tissue lysates; Mouse embryo and heart tissue lysates; Rat heart and brain tissue lysates; SH-SY5Y and Neuro-2a whole cell lysates. IHC-P: Human cerebrum and testis tissue; Mouse testis tissue; Rat testis tissue.

  • General notes

    ab274426 is the carrier-free version of ab274417. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23301-10

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] - BSA and Azide free (ab274426)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] - BSA and Azide free (ab274426)

    This data was developed using ab274417, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Neurturin with ab274417 at 1/500 (1.094 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse testis. The section was incubated with ab274417 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] - BSA and Azide free (ab274426)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] - BSA and Azide free (ab274426)

    This data was developed using ab274417, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Neurturin with ab274417 at 1/500 (1.094 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum (PMID: 25841760). The section was incubated with ab274417 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Neurturin antibody [EPR23301-10] - BSA and Azide free (ab274426)
    Western blot - Anti-Neurturin antibody [EPR23301-10] - BSA and Azide free (ab274426)
    All lanes : Anti-Neurturin antibody [EPR23301-10] (ab274417) at 1/1000 dilution

    Lane 1 : Mouse embryo tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
    Lane 4 : Rat heart tissue lysate
    Lane 5 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 22 kDa
    Observed band size: 16,28 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab274417, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lanes 1-3 were developed using a higher sensitivity ECL substrate.

    16-kDa Monomeric and 28-kDa dimeric form of Neurturin are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 15829225, PMID: 24067900).

    Exposure times: Lane 1-3: 3 minutes Lane 4-5: 37 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] - BSA and Azide free (ab274426)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] - BSA and Azide free (ab274426)

    This data was developed using ab274417, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Neurturin with ab274417 at 1/500 (1.094 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human testis. The section was incubated with ab274417 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Neurturin antibody [EPR23301-10] - BSA and Azide free (ab274426)
    Western blot - Anti-Neurturin antibody [EPR23301-10] - BSA and Azide free (ab274426)
    All lanes : Anti-Neurturin antibody [EPR23301-10] (ab274417) at 1/1000 dilution

    Lane 1 : Human heart tissue lysate
    Lane 2 : Human testis tissue lysate
    Lane 3 : Human brain tissue lysate
    Lane 4 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 16,28 kDa why is the actual band size different from the predicted?



    This data was developed using ab274417, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    16-kDa Monomeric and 28-kDa dimeric form of Neurturin are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 15829225, PMID: 24067900).

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] - BSA and Azide free (ab274426)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] - BSA and Azide free (ab274426)

    This data was developed using ab274417, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Neurturin with ab274417 at 1/500 (1.094 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat testis (PMID: 10415380). The section was incubated with ab274417 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Neurturin antibody [EPR23301-10] - BSA and Azide free (ab274426)
    Anti-Neurturin antibody [EPR23301-10] - BSA and Azide free (ab274426)

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