Cathepsin S Inhibitor Assay Kit (Fluorometric) (ab185437)
Key features and details
- Assay type: Semi-quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Inhibitor compounds
Overview
-
Product name
Cathepsin S Inhibitor Assay Kit (Fluorometric)
See all Cathepsin S kits -
Detection method
Fluorescent -
Sample type
Inhibitor compounds -
Assay type
Semi-quantitative -
Product overview
Cathepsin S Inhibitor Screening Kit (ab185437) is an assay that uses the ability of Cathepsin S to cleave the synthetic AFC based peptide substrate to release AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin S inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This high-throughput adaptable assay kit is simple, sensitive and rapid tool to screen the potential inhibitors of Cathepsin S.
Visit our FAQs page for tips and troubleshooting.
-
Notes
Cathepsin S (CTSS, EC 3.4.22.27) is a lysosomal cysteine proteinase that is suggested to participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules
-
Platform
Microplate reader
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Cathepsin S 1 vial CTSS Inhibitor (Z-FF-FMK 1 mM) 1 x 20µl CTSS Reaction Buffer 1 x 15ml CTSS Substrate (Z-VVR-AFC 10 mM) 1 x 0.2ml -
Research areas
-
Relevance
The cathepsin family of proteases contains several diverse classes of enzymes. The cysteine protease class comprises cathepsin B, H, K, L, O, and S. The aspartyl protease class contains cathepsin D and E. Cathepsin G belongs to the serine protease class. Cathepsins are involved in various cellular events such as peptide biosynthesis, protein degradation, and apoptosis. Cathepsin S has been shown to be able to function as an elastase over a broad pH range in alveolar macrophages. -
Cellular localization
Lysosome -
Alternative names
- CathepsinS
- CTSS
- MGC3886
Images
-
Inhibition of Cathepsin S activity by the CTSS Inhibitor [Z-FF-FMK]
Inhibition of Cathepsin S activity by Cathepsin S Inhibitor Screening Kit was performed following kit protocol. This is example data only.
