Anti-SV40 VP2 + VP3 antibody (ab53983)
Key features and details
- Rabbit polyclonal to SV40 VP2 + VP3
- Suitable for: WB
- Reacts with: Simian Virus 40
- Isotype: IgG
Overview
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Product name
Anti-SV40 VP2 + VP3 antibody -
Description
Rabbit polyclonal to SV40 VP2 + VP3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Simian Virus 40 -
Immunogen
Recombinant fusion protein: MAVDLYRPDD YYDILFPGVQ TFVHSVQYLD PRHWGPTLFN AISQAFWRVI QNDIPRLTSQ ELERRTQRYL RDSLARFLEE TTWTVINAPV NWYNSLQDYY STLSPIRPTM VRQVANREGL QISFGHTYDN IDEADSIQQV TERWEAQSQS PNVQSGEFIE KFEAPGGANQ RTAPQWMLPL LLGLYGSVTS ALKAYEDGPN KKKRKLSRGS SQKTKGTSAS AKARHKRRNR SSRS
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General notes
This antibody recognises two of the SV40 capsid proteins - VP2 and VP3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: Whole serum, 50% Glycerol -
Concentration information loading... -
Purity
Whole antiserum -
Primary antibody notes
This antibody recognises two of the SV40 capsid proteins - VP2 and VP3 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SV40 VP2 + VP3 antibody (ab53983) This image is courtesy of an anonymous AbreviewAll lanes : Anti-SV40 VP2 + VP3 antibody (ab53983) at 1/5000 dilution
Lane 1 : BK virus infected 293T cell lysate
Lane 2 : Mock 293T cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38,26 kDa
Observed band size: 27,38 kDa why is the actual band size different from the predicted?
Exposure time: 6 minutes
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Western blot - Anti-SV40 VP2 + VP3 antibody (ab53983)SV40 was ultracentrifuged on CsCl-gradient, fractions were collected from the top of the tube, run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fractions 3,4 and 6 were run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fraction 3,4 and 6 were run on PAGe and analysed by Western blot with α-VP1 and α-VP2/3
