Antibodies, Proteins, Kits and Reagents for Life Science

251 280 ₸ Product size

100 µl 251 280 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ICAM3 antibody [EPR3994-123] (ab109405)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3994-123] to ICAM3
Suitable for: ICC, WB, IHC-P
Reacts with: Human

Product name

Anti-ICAM3 antibody [EPR3994-123]
See all ICAM3 primary antibodies
Description

Rabbit monoclonal [EPR3994-123] to ICAM3
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- U937, HeLa, Jurkat, HuT-78, Ramos, and THP-1 cell lysates; Human spleen, colon and tonsil tissue.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3994-123
Isotype

IgG
Research areas

Western blot - Anti-ICAM3 antibody [EPR3994-123] (ab109405)

All lanes : Anti-ICAM3 antibody [EPR3994-123] (ab109405) at 1/1000 dilution

Lane 1 : U937 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HuT-78 cell lysate
Lane 4 : Ramos cell lysate
Lane 5 : THP-1 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 60 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)

Immunohistochemical analysis of ICAM3 in paraffin-embedded Human spleen tissue using ab109405 at 1/500 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry - Anti-ICAM3 antibody [EPR3994-123] (ab109405)

This data was developed using the same antibody clone in a different buffer formulation (ab247851).
Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) labeling ICAM3 with purified ab247851 at 1/500 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.24 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)

Immunohistochemical analysis of ICAM3 in paraffin-embedded Human colon tissue using ab109405 at 1/500 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)

Immunohistochemical analysis of ICAM3 in paraffin-embedded Human tonsil tissue using ab109405 at 1/500dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-ICAM3 antibody [EPR3994-123] (ab109405)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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