Anti-ICAM3 antibody [ICAM3.1] (ab10804)
![Anti-ICAM3 antibody [ICAM3.1] (ab10804)](https://www.abcam.com/ps/products/10/ab10804/Images/ab10804-18465-anti-icam3-antibody-icam31-immunohistochemistry.jpg "Anti-ICAM3 antibody [ICAM3.1] (ab10804)")
Key features and details
- Mouse monoclonal [ICAM3.1] to ICAM3
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-ICAM3 antibody [ICAM3.1]
See all ICAM3 primary antibodies -
Description
Mouse monoclonal [ICAM3.1] to ICAM3 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
ICAM 3Fc chimeric fusion protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
ICAM3.1 -
Myeloma
Sp2 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [ICAM3.1] (ab10804)Ab10804 staining Human malignant stomach. Staining is localised to the membrane.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
