Anti-pan Cytokeratin antibody [PCK-26] (ab6401)
Key features and details
- Mouse monoclonal [PCK-26] to pan Cytokeratin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-pan Cytokeratin antibody [PCK-26]
See all pan Cytokeratin primary antibodies -
Description
Mouse monoclonal [PCK-26] to pan Cytokeratin -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Full length native protein (purified) corresponding to Human pan Cytokeratin.
-
General notes
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
PCK-26 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Images
-
Lane 1 : Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 2.5 µg/ml
Lane 2 : Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 5 µg/ml
Lane 3 : Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 10 µg/ml
All lanes : HeLa cell lysate
Secondary
All lanes : Peroxidase-conjugated goat anti-mouse IgGThis image was generated using the ascites version of the product.
-
All lanes: pan Cytokeratin antibody (ab6401) at 1/1000 dilution + whole cell lysate of Primary mouse pancreatic cancer cell lines (35 µg)
Secondary: An HRP-conjugated Sheep anti-mouse IgG polyclonal (1/5000) developed using the ECL technique
Performed under non-reducing conditions.
Blocking Step: 10% Milk for 1 hour at room temperatureThis image was generated using the ascites version of the product.