Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (ab215838)

Key features and details

  • Mouse monoclonal [PAN-CK (Cocktail)] to pan Cytokeratin
  • Suitable for: IHC-P, Flow Cyt, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)]
    See all pan Cytokeratin primary antibodies

  • Description

    Mouse monoclonal [PAN-CK (Cocktail)] to pan Cytokeratin

  • Host species

    Mouse

  • Specificity

    This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) Cytokeratins, with 67kDa (Cytokeratin1); 64kDa (Cytokeratin3); 59kDa (Cytokeratin4); 58kDa (Cytokeratin5); 56kDa (Cytokeratin6); 55kDa (Cytokeratin7); 52kDa (Cytokeratin8); 56.5kDa (Cytokeratin10); 53kDa (Cytokeratin13); 50kDa (Cytokeratin14); 50kDa (Cytokeratin15); 48kDa (Cytokeratin16); 46kDa (Cytokeratin17); 45kDa (Cytokeratin18) and 40kDa (Cytokeratin19).

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Full length native protein (purified) corresponding to Human pan Cytokeratin. Human epidermal keratins.

  • Positive control

    • IHC-P: Human basal cell carcinoma and squamous cell carcinoma tissues. ICC and Flow Cyt: HeLa cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    ab215838 is purified from Bioreactor Concentrate by Protein A/G.

  • Clonality

    Monoclonal

  • Clone number

    PAN-CK (Cocktail)

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (ab215838)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (ab215838)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human basal cell carcinoma tissue labelling pan Cytokeratin with ab215838 at 2 µg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.

  • Immunocytochemistry - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (ab215838)
    Immunocytochemistry - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (ab215838)
    Immunofluorescence analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling pan Cytokeratin with ab215838 at 2 µg/mL followed by Goat anti-Mouse IgG-CF488 (cyan). The nuclear counterstain is NucSpot (red).
  • Flow Cytometry - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (ab215838)
    Flow Cytometry - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (ab215838)
    Flow cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling pan-Cytokeratin with ab215838 at 2µg/10^6 cells followed by Goat Anti-Mouse IgG-CF488 (blue); isotype control (red).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (ab215838)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)] (ab215838)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human squamous cell carcinoma tissue labelling pan Cytokeratin with ab215838 at 2 µg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.

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