Overlay histogram showing A431 cells stained with ab110038 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110038, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

ab110038 at 1/200 dilution staining p63 in paraffin-embedded ovarian cancer tissue by Immunohistochemistry. Detection utilised DAB staining.

All lanes : Anti-p63 antibody [4E5] (ab110038) at 1/500 dilution

Lane 1 : A431 cell lysate.
Lane 2 : HeLa cell lysate.
Lane 3 : Jurkat cell lysate.
Lane 4 : THP1 cell lysate.
Lane 5 : NIH 3T3 cell lysate.
Lane 6 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate.
Lane 7 : PC12 cell lysate.

Predicted band size: 77 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?

ab110038 should be used at a proposed dilution of 1/10000 for ELISA.

ab110038 at 1/200 dilution staining p63 in paraffin-embedded lung cancer tissue by Immunohistochemistry. Detection utilised DAB staining.