Anti-p63 antibody [4E5] (ab110038)
Key features and details
- Mouse monoclonal [4E5] to p63
- Suitable for: Flow Cyt, WB, ELISA, IHC-P
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-p63 antibody [4E5]
See all p63 primary antibodies -
Description
Mouse monoclonal [4E5] to p63 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human -
Immunogen
Synthetic peptide of Human p63.
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Positive control
- A431, HeLa, Jurkat, THP1, NIH 3T3, COS7 and PC12 cell lysates. Ovarian cancer and lung cancer tissue.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR118984-25 are from Tissue Culture Supernatant
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
4E5 -
Isotype
IgG1 -
Research areas
Images
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Overlay histogram showing A431 cells stained with ab110038 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110038, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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ab110038 at 1/200 dilution staining p63 in paraffin-embedded ovarian cancer tissue by Immunohistochemistry. Detection utilised DAB staining.
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All lanes : Anti-p63 antibody [4E5] (ab110038) at 1/500 dilution
Lane 1 : A431 cell lysate.
Lane 2 : HeLa cell lysate.
Lane 3 : Jurkat cell lysate.
Lane 4 : THP1 cell lysate.
Lane 5 : NIH 3T3 cell lysate.
Lane 6 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate.
Lane 7 : PC12 cell lysate.
Predicted band size: 77 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
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ab110038 should be used at a proposed dilution of 1/10000 for ELISA.
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ab110038 at 1/200 dilution staining p63 in paraffin-embedded lung cancer tissue by Immunohistochemistry. Detection utilised DAB staining.