Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p63 antibody [EPR5701] - BSA and Azide free (ab214790)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5701] to p63 - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-p63 antibody [EPR5701] - BSA and Azide free
    See all p63 primary antibodies

  • Description

    Rabbit monoclonal [EPR5701] to p63 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human p63 aa 1-250. The exact sequence is proprietary.
    Database link: Q9H3D4

  • Positive control

    • WB: Rat bladder and skin, Mouse bladder, skin and thymus tissue lysates; HaCaT, PC-12, and A431 cell lysates; IHC-P: Human breast tissue; ICC/IF: A431 and primary corneal limbal cells; Flow cyt: A431 cells.

  • General notes

    Ab214790 is the carrier-free version of ab124762. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab214790 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-p63 antibody [EPR5701] - BSA and Azide free (ab214790)
    Western blot - Anti-p63 antibody [EPR5701] - BSA and Azide free (ab214790)
    All lanes : Anti-p63 antibody [EPR5701] (ab124762) at 1/1000 dilution (Purified)

    Lane 1 : HaCaT (Human skin keratinocyte) whole cell lysates
    Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates
    Lane 3 : Mouse skin lysates
    Lane 4 : Mouse thymus lysates
    Lane 5 : Mouse bladder lysates
    Lane 6 : Rat skin lysates
    Lane 7 : Rat bladder lysates
    Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 77 kDa
    Observed band size: 37-75 kDa
    why is the actual band size different from the predicted?



    The bands observed are consistent with what have been described in PMID 30649915 as isoforms of p63.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124762).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [EPR5701] - BSA and Azide free (ab214790)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [EPR5701] - BSA and Azide free (ab214790)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling p63 with Purified ab124762 at 1/5000 dilution (0.16 µg/mL). Heat mediated antigen retrieval using Bond™™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124762).

  • Immunocytochemistry - Anti-p63 antibody [EPR5701] - BSA and Azide free (ab214790)
    Immunocytochemistry - Anti-p63 antibody [EPR5701] - BSA and Azide free (ab214790)

    Immunocytochemistry/ Immunofluorescence analysis of A431(Human epidermoid carcinoma epithelial cell) cells labeling p63 with Purified ab124762 at 1/200 dilution (4 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124762).

  • Flow Cytometry - Anti-p63 antibody [EPR5701] - BSA and Azide free (ab214790)
    Flow Cytometry - Anti-p63 antibody [EPR5701] - BSA and Azide free (ab214790)

    Clone EPR5701 (ab214790) has been successfully conjugated by Abcam. This image was generated using Anti-p63 antibody [EPR5701] (Alexa Fluor® 647). Please refer to ab246728 for protocol details.

    Overlay histogram showing A431 cells stained with ab246728 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal Goat serum to block non-specific protein-protein interactions followed by the antibody (ab246728) (1x 106 cells in 100 µl at 0.08 µg/ml (1/6250 dilution)) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

    This antibody gave a positive signal in A431 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • Immunocytochemistry - Anti-p63 antibody [EPR5701] - BSA and Azide free (ab214790)
    Immunocytochemistry - Anti-p63 antibody [EPR5701] - BSA and Azide free (ab214790)

    Clone EPR5701 (ab214790) has been successfully conjugated by Abcam. This image was generated using Anti-p63 antibody [EPR5701] (Alexa Fluor® 488). Please refer to ab246727 for protocol details.

    ab246727 staining p63 in A431 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab246727 at 1/100 dilution (shown in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in A431 cells fixed with 4% formaldehyde (10 min).

  • Flow Cytometry - Anti-p63 antibody [EPR5701] - BSA and Azide free (ab214790)
    Flow Cytometry - Anti-p63 antibody [EPR5701] - BSA and Azide free (ab214790)
    Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling p63 with Purified ab124762 at 1/80 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124762).
  • Immunocytochemistry - Anti-p63 antibody [EPR5701] - BSA and Azide free (ab214790)
    Immunocytochemistry - Anti-p63 antibody [EPR5701] - BSA and Azide free (ab214790) This image is courtesy of an Abreview submitted by Manuel Chacon.

    Unpurified ab124762 staining p63 in Human corneal limbal epithelial cells (primary culture) by ICC/IF (immunocytochemistry/immunofluorescence). Cells were fixed with methanol and permeabilized with 0.3% Triton X-100 for 5 minutes. Samples were incubated with primary antibody (1/100 in PBS + 10% Goat serum) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124762).

  • Anti-p63 antibody [EPR5701] - BSA and Azide free (ab214790)
    Anti-p63 antibody [EPR5701] - BSA and Azide free (ab214790)

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