Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)
![Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)](https://www.abcam.com/ps/products/249/ab249325/Images/ab249325-410370-anti-p53-phospho-s20-antibody-epr2156-2-bsa-and-azide-free-western-blot.jpg "Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2156(2)] to p53 (phospho S20) - BSA and Azide free
- Suitable for: Dot blot, WB, IP
- Reacts with: Human
Overview
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Product name
Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free
See all p53 primary antibodies -
Description
Rabbit monoclonal [EPR2156(2)] to p53 (phospho S20) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody only detects p53 phosphorylated at Serine 20.
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Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab249325 is the carrier-free version of ab157454. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249325 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR2156(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)All lanes : Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454) at 1/1000 dilution
Lane 1 : T-47D (human mammary gland ductal carcinoma) treated with Etoposide whole cell lysate
Lane 2 : Untreated T47D (human mammary gland ductal carcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 44 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteThis data was developed using ab157454, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)](https://www.abcam.com/ps/products/249/ab249325/Images/ab249325-408373-anti-p53-phospho-s20-antibody-epr2156-2-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)This data was developed using ab157454&
44; the same antibody clone in a different buffer formulation.
>Detection of p53 Western Blot of Immunprecipitate. T47D cells treated with etoposide lysate immunoprecipitated using ab157454 at 1/10 dilution followed by HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG.
Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454) at 1/1000 dilution + Immunoprecipitation pellet from T47-D cells treated with etoposide lysate at 10 µg
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![Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)](https://www.abcam.com/ps/products/249/ab249325/Images/ab249325-405784-anti-p53-phospho-s20-antibody-epr2156-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)All lanes : Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454) at 1/1000 dilution
Lane 1 : T47-D cell lysate
Lane 2 : T47D cell lysate treated with etoposide.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 44 kDaThis data was developed using ab157454, the same antibody clone in a different buffer formulation.
Lower panel shows labeling with an anti-p53 C terminal antibody.
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![Dot Blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)](https://www.abcam.com/ps/products/249/ab249325/Images/ab249325-1-anti-p53-phospho-s20-antibody-epr2156-2-bsa-and-azide-free-dot-blot.jpg)
Dot Blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)This data was developed using ab157454, the same antibody clone in a different buffer formulation.Primary antibody dilution: 1/1000 Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated Secondary antibody dilution: 1/2500 Blocking & dilution buffer: 5% NFDM/TBST Lane 1 sample: p53 (pS20) phospho peptide Lane 2 sample: p53 non-phospho peptide Exposure time: 10 seconds -
![Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)](https://www.abcam.com/ps/products/249/ab249325/Images/ab249325-5-benefits-of-recombinant-antibodies.png)
Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)