Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2156(2)] to p53 (phospho S20) - BSA and Azide free
  • Suitable for: Dot blot, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free
    See all p53 primary antibodies

  • Description

    Rabbit monoclonal [EPR2156(2)] to p53 (phospho S20) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects p53 phosphorylated at Serine 20.

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249325 is the carrier-free version of ab157454. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249325 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)
    Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)
    All lanes : Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454) at 1/1000 dilution

    Lane 1 : T-47D (human mammary gland ductal carcinoma) treated with Etoposide whole cell lysate
    Lane 2 : Untreated T47D (human mammary gland ductal carcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 53 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This data was developed using ab157454, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)
    Immunoprecipitation - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)

    This data was developed using ab157454&

    44; the same antibody clone in a different buffer formulation.

    >Detection of p53 Western Blot of Immunprecipitate. T47D cells treated with etoposide lysate immunoprecipitated using ab157454 at 1/10 dilution followed by HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG.

    Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454) at 1/1000 dilution + Immunoprecipitation pellet from T47-D cells treated with etoposide lysate at 10 µg
  • Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)
    Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)
    All lanes : Anti-p53 (phospho S20) antibody [EPR2156(2)] (ab157454) at 1/1000 dilution

    Lane 1 : T47-D cell lysate
    Lane 2 : T47D cell lysate treated with etoposide.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 44 kDa



    This data was developed using ab157454, the same antibody clone in a different buffer formulation.

    Lower panel shows labeling with an anti-p53 C terminal antibody.

  • Dot Blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)
    Dot Blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)
    This data was developed using ab157454, the same antibody clone in a different buffer formulation.Primary antibody dilution: 1/1000 Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated Secondary antibody dilution: 1/2500 Blocking & dilution buffer: 5% NFDM/TBST Lane 1 sample: p53 (pS20) phospho peptide Lane 2 sample: p53 non-phospho peptide Exposure time: 10 seconds
  • Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)
    Anti-p53 (phospho S20) antibody [EPR2156(2)] - BSA and Azide free (ab249325)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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