Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p53 antibody [EPR20416-120] (ab246550)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20416-120] to p53
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-p53 antibody [EPR20416-120]
    See all p53 primary antibodies

  • Description

    Rabbit monoclonal [EPR20416-120] to p53

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: LLC1 whole cell lysate. ICC/IF: LLC1 cells. Flow Cyt: LLC1 cells. IP: LLC1 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunoprecipitation - Anti-p53 antibody [EPR20416-120] (ab246550)
    Immunoprecipitation - Anti-p53 antibody [EPR20416-120] (ab246550)

    p53 was immunoprecipitated from 0.35 mg of LLC1 (mouse lung carcinoma cell line) whole cell lysate with ab246550 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab246550 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: LLC1 whole cell lysate 10 µg (Input). 

    Lane 2: ab246550 IP in LLC1 whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246550 in LLC1 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Immunocytochemistry/ Immunofluorescence - Anti-p53 antibody [EPR20416-120] (ab246550)
    Immunocytochemistry/ Immunofluorescence - Anti-p53 antibody [EPR20416-120] (ab246550)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LLC1 (mouse lung carcinoma cell line) cells labeling p53 with ab246550 at 1/100 dilution followed by a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in LLC1 cells is observed.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with an Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow Cytometry - Anti-p53 antibody [EPR20416-120] (ab246550)
    Flow Cytometry - Anti-p53 antibody [EPR20416-120] (ab246550)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized LLC1 (mouse lung carcinoma cell line) cell line labeling p53 with ab246550 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-p53 antibody [EPR20416-120] (ab246550)
    Western blot - Anti-p53 antibody [EPR20416-120] (ab246550)
    Anti-p53 antibody [EPR20416-120] (ab246550) at 1/1000 dilution + LLC1 (mouse lung carcinoma cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 43 kDa
    Observed band size: 53 kDa why is the actual band size different from the predicted?


    Exposure time: 92 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 27702985).

  • Anti-p53 antibody [EPR20416-120] (ab246550)
    Anti-p53 antibody [EPR20416-120] (ab246550)

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