Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (ab228877)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP233(2)Y] to p27 KIP 1 (phospho S10) - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, Dot blot
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free
    See all p27 KIP 1 primary antibodies

  • Description

    Rabbit monoclonal [EP233(2)Y] to p27 KIP 1 (phospho S10) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IP, IHC-P, Dot blotmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Monkey

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: MCF7 whole cell lysate; IHC: Human colon cancer tissue; WB: HeLa, PC-12, and NIH/3T3.

  • General notes

    Ab228877 is the carrier-free version of ab62364. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab228877 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (ab228877)
    Western blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (ab228877)
    All lanes : Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, membrane treated with Alkaline Phosphatase for 1 hour
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, membrane treated with Alkaline Phosphatase for 1 hour
    Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysate, membrane treated with Alkaline Phosphatase for 1 hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa



    This data was developed using ab62364, the same antibody clone in a different buffer formulation.

    Blocking buffer: 5% NFDM/TBST

  • Immunohistochemistry paraffin embedded sections - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (ab228877)
    Immunohistochemistry paraffin embedded sections - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (ab228877)
    This data was developed using ab62364 the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling p27 KIP 1 with Purified ab62364 at 1:100 dilution (4.19 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunoprecipitation - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (ab228877)
    Immunoprecipitation - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (ab228877)

    This data was developed using ab62364, the same antibody clone in a different buffer formulation.

    Purified ab62364 at 1/50 dilution (2µg) immunoprecipitating p27 KIP 1 in MCF7 whole cell lysate.
    Lane 1 (input): MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab62364 + MCF7 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab62364 in MCF7 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 27 kDa

  • Dot Blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (ab228877)
    Dot Blot - Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - BSA and Azide free (ab228877)

    Dot blot analysis of p27 KIP 1 (pS10) phospho peptide (Lane 1) and p27 KIP 1 non-phospho peptide (Lane 2) using ab62364 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.

    Blocking and Diluting buffer and concentration: 5% NFDM /TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62364).

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