Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p23 antibody [EPR3846] (ab92503)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3846] to p23
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-p23 antibody [EPR3846]
    See all p23 primary antibodies

  • Description

    Rabbit monoclonal [EPR3846] to p23

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human p23 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: HEK-293T, HeLa, Human spleen, Jurkat and HepG2 cell lysates ICC/IF: HeLa cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-p23 antibody [EPR3846] (ab92503)
    Western blot - Anti-p23 antibody [EPR3846] (ab92503)
    All lanes : Anti-p23 antibody [EPR3846] (ab92503) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : PTGES3 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 19 kDa
    Observed band size: 23 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab92503 observed at 23 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab92503 Anti-p23 antibody [EPR3846] was shown to specifically react with p23 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266791 (knockout cell lysate ab258151) was used. Wild-type and p23 knockout samples were subjected to SDS-PAGE. ab92503 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-p23 antibody [EPR3846] (ab92503)
    Western blot - Anti-p23 antibody [EPR3846] (ab92503)
    All lanes : Anti-p23 antibody [EPR3846] (ab92503) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human spleen lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 19 kDa
    Observed band size: 23 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-p23 antibody [EPR3846] (ab92503)
    Immunocytochemistry/ Immunofluorescence - Anti-p23 antibody [EPR3846] (ab92503)
    Immunofluorescent staining of HeLa cells, using ab92503 at a dilution of 1/100.
  • Anti-p23 antibody [EPR3846] (ab92503)
    Anti-p23 antibody [EPR3846] (ab92503)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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