Antibodies, Proteins, Kits and Reagents for Life Science

308 236 ₸ Product size

100 µl 308 236 ₸ 10 µl 123 964 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3846] to p23
Suitable for: WB, ICC/IF
Knockout validated
Reacts with: Human

Product name

Anti-p23 antibody [EPR3846]
See all p23 primary antibodies
Description

Rabbit monoclonal [EPR3846] to p23
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human p23 aa 50-150. The exact sequence is proprietary.
Positive control
- WB: HEK-293T, HeLa, Human spleen, Jurkat and HepG2 cell lysates ICC/IF: HeLa cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3846
Isotype

IgG
Research areas

Western blot - Anti-p23 antibody [EPR3846] (ab92503)

All lanes : Anti-p23 antibody [EPR3846] (ab92503) at 1/1000 dilution

Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : PTGES3 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 19 kDa
Observed band size: 23 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab92503 observed at 23 kDa. Red - loading control ab8245 observed at 36 kDa.

ab92503 Anti-p23 antibody [EPR3846] was shown to specifically react with p23 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266791 (knockout cell lysate ab258151) was used. Wild-type and p23 knockout samples were subjected to SDS-PAGE. ab92503 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-p23 antibody [EPR3846] (ab92503)

All lanes : Anti-p23 antibody [EPR3846] (ab92503) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Human spleen lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 19 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-p23 antibody [EPR3846] (ab92503)

Immunofluorescent staining of HeLa cells, using ab92503 at a dilution of 1/100.
Anti-p23 antibody [EPR3846] (ab92503)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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