Mouse alpha 1 Fetoprotein Matched Antibody Pair Kit (ab216056)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 250 pg/ml - 16000 pg/ml
Overview
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Product name
Mouse alpha 1 Fetoprotein Matched Antibody Pair Kit
See all alpha 1 Fetoprotein kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
98.8 pg/ml -
Range
250 pg/ml - 16000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse alpha 1 Fetoprotein Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse alpha 1 Fetoprotein.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab210969. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse alpha 1 Fetoprotein Capture Antibody 2 x 50µg 1 x 50µg Mouse alpha 1 Fetoprotein Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse alpha 1 Fetoprotein Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. -
Tissue specificity
Plasma. Synthesized by the fetal liver and yolk sac. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Developmental stage
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma. -
Post-translational
modificationsIndependent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Afp
- AFPD
- Alpha fetoglobulin
see all -
Database links
- Entrez Gene: 11576 Mouse
- SwissProt: P02772 Mouse
- Unigene: 358570 Mouse
Images
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Mouse alpha Fetoprotein standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse alpha 1 Fetoprotein Matched Antibody Pair Kit (ab216056)To learn more about the advantages of recombinant antibodies see here.
