Mouse alpha Fetoprotein ELISA Kit (ab210969)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 287 pg/ml
- Range: 0.94 ng/ml - 60 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
-
Product name
Mouse alpha Fetoprotein ELISA Kit
See all alpha 1 Fetoprotein kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 5 3% Inter-assay Sample n Mean SD CV% serum 3 1.6% -
Sample type
Cell culture supernatant, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
287 pg/ml -
Range
0.94 ng/ml - 60 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 99 98% - 101% Urine 99 96% - 101% Serum 120 117% - 123% Hep Plasma 119 116% - 125% EDTA Plasma 102 98% - 108% Cit plasma 110 108% - 113% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse alpha Fetoprotein ELISA Kit (ab210969) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of alpha Fetoprotein protein in cell culture supernatant, plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse alpha Fetoprotein with 287 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
Alpha fetoprotein is a secreted single chain glycoprotein expressed normally during fetal development as well as by liver pathologies, including cancer. It is a member of the albuminoid superfamily and may act as a carrier protein. Pregnant mice may have elevated alpha -fetoprotein levels derived from fetal circulation; male and female adults have low levels of circulating alpha -fetoprotein
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse alpha Fetoprotein Capture Antibody 1 x 600µl 10X Mouse alpha Fetoprotein Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Mouse alpha Fetoprotein Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. -
Tissue specificity
Plasma. Synthesized by the fetal liver and yolk sac. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Developmental stage
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma. -
Post-translational
modificationsIndependent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- Afp
- AFPD
- Alpha fetoglobulin
see all -
Database links
- Entrez Gene: 11576 Mouse
- SwissProt: P02772 Mouse
- Unigene: 358570 Mouse
Images
-
Other - Mouse alpha Fetoprotein ELISA Kit (ab210969)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of mouse alpha Fetoprotein standard curve.Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native alpha Fetoprotein in mouse serum and plasma samples.The concentrations of alpha Fetoprotein were measured in duplicates, interpolated from the alpha Fetoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 50%, and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha Fetoprotein concentration was determined to be 35.2 ng/mL in serum, 57.4 ng/mL in plasma (EDTA), 16.6 ng/mLin plasma (heparin), and 17.3 ng/mL in plasma (citrate).
-
Interpolated concentrations of spike alpha Fetoprotein in mouse cell culture supernatant and urine samples.The concentrations of alpha Fetoprotein were measured in duplicates, interpolated from the alpha Fetoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture supernatant 50% and urine 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
-
Interpolated concentrations of native alpha Fetoprotein in mouse serum samples.The concentrations of alpha Fetoprotein were measured in duplicates, interpolated from the alpha Fetoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows: normal serum 50% and pregnant serum 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha Fetoprotein concentration was determined to be 35.2 ng/mL in normal serum and 7226 ng/mL in pregnant mouse serum.
-
Sandwich ELISA - Mouse alpha Fetoprotein ELISA Kit (ab210969)To learn more about the advantages of recombinant antibodies see here.
