Recombinant Human IGF2BP2/IMP-2 protein (ab173053)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Tags: His tag C-Terminus
- Suitable for: HPLC, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: Phosphate Buffer, 0.877% Sodium chloride
Supplied as a 0.2 µM filtered solution -
ReconstitutionAlways centrifuge tubes before opening. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in ddH2O. Aliquot the reconstituted solution to minimize freeze-thaw cycles. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at
General Info
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Alternative names
- Hepatocellular carcinoma autoantigen p62
- IF2B2_HUMAN
- IGF 2 mRNA binding protein 2
see all -
Function
Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs. -
Tissue specificity
Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis. -
Sequence similarities
Belongs to the RRM IMP/VICKZ family.
Contains 4 KH domains.
Contains 2 RRM (RNA recognition motif) domains. -
Domain
The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA. -
Cellular localization
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab173053 has not yet been referenced specifically in any publications.
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