Monkey Albumin ELISA Kit (ab190533)
Key features and details
- Sensitivity: 1.795 ng/ml
- Range: 12.5 ng/ml - 400 ng/ml
- Sample type: Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Monkey
Overview
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Product name
Monkey Albumin ELISA Kit
See all Albumin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.795 ng/ml -
Range
12.5 ng/ml - 400 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum >=85 12.5ng/ml - 400ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Monkey -
Product overview
Abcam’s Albumin Monkey ELISA kit (ab190533) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Albumin in monkey serum, plasma and urine.
In this assay the Albumin present in samples reacts with the anti-Albumin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Albumin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Albumin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Albumin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Albumin in the test sample. The quantity of Albumin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Notes
Albumin (Alb) is a polyfunctional protein contributing to homeostasis through mechanisms of hemodynamics, transport and nutrition. Albumin is found both intra and extravascularly in all mammals and many lower vertebrates. It is a molecule of about 67,000 daltons, synthesized by the liver. Normally only very trace amounts of albumin escape reabsorption by kidney glomeruli and is excreted into the urine. Many occult diseases can cause kidney damage which may result in excessive amounts of serum proteins, including albumin, to be excreted by the kidney and into the urine. This ELISA kit can be used to measure albumin in serum, tissue extracts and other biological fluids.
The calibrator used in this kit is of human origin. It is quantitated with purified Cynomolgus monkey albumin and behaves identically with it.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Albumin Monkey Antibody coated microwells 1 unit Albumin Monkey HRP Conjugate 1 vial Chromogen Substrate Solution 1 x 12ml Lyophilized Albumin Monkey Calibrator 1 vial Stop Solution 1 x 12ml -
Research areas
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Function
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Post-translational
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- alb
- ALBU_HUMAN
- Albumin (32 AA)
see all
Images
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Standard Curve
Representative standard curve using ab190533 Albumin Monkey ELISA Kit.