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Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2389(2)] to p130 (phospho T986)
  • Suitable for: WB, IP, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-p130 (phospho T986) antibody [EPR2389(2)]
    See all p130 primary antibodies

  • Description

    Rabbit monoclonal [EPR2389(2)] to p130 (phospho T986)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Mouse
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat and NIH/3T3 whole cell lysates. Flow Cyt: Jurkat cells. IP: NIH/3T3 whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928)
    Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928)
    All lanes : Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928) at 1/1000 dilution

    Lane 1 : Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate treated with phosphatase on the membrane

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 128 kDa
    Observed band size: 128 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 2% BSA.

  • Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928)
    Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928)
    All lanes : Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928) at 1/1000 dilution

    Lane 1 : Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate treated with phosphatase on the membrane

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 128 kDa
    Observed band size: 128 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928)
    Western blot - Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928)
    All lanes : Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928) at 1/1000 dilution

    Lane 1 : Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with single phospho peptide
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with non phospho peptide

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 128 kDa
    Observed band size: 128 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928)
    Flow Cytometry - Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928)

    Flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling p130 (phospho T986) with ab211928 at 1/200 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928)
    Immunoprecipitation - Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928)

    p130 (phospho T986) was immunoprecipitated from 0.35 mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab211928 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab211928 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: NIH/3T3 whole cell lysate 10µg (Input).

    Lane 2: ab211928 IP in NIH/3T3 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211928 in NIH/3T3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

  • Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928)
    Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928)

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