Anti-p130 (phospho S672) antibody [EP2126Y] (ab76255)
Key features and details
- Rabbit monoclonal [EP2126Y] to p130 (phospho S672)
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-p130 (phospho S672) antibody [EP2126Y]
See all p130 primary antibodies -
Description
Rabbit monoclonal [EP2126Y] to p130 (phospho S672) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human p130 (phospho S672). The exact sequence is proprietary.
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Positive control
- WB: Jurkat whole cell lysate (ab7899), treated or untreated with AP. IP: Jurkat was serum starved for 24h, then treated with 10% FBS for 20h whole cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2126Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-p130 (phospho S672) antibody [EP2126Y] (ab76255) at 1/10000 dilution
Lane 1 : Jurkat cell lysate, treated with AP
Lane 2 : Jurkat cell lysate, untreated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-rabbit (HRP) at 1/2000 dilution
Predicted band size: 128 kDa
Observed band size: 128 kDaControl: Rabbit monoclonal [EP2141Y] to p130 (ab76234)
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Purified ab76255 at 1/20 dilution (1µg) immunoprecipitating p130 in Jurkat was serum starved for 24h, then treated with 10% FBS for 20h whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) was serum starved for 24h, then treated with 10% FBS for 20h whole cell lysate 10µg
Lane 2 (+): ab76255 + Jurkat was serum starved for 24h, then treated with 10% FBS for 20h whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76255 in Jurkat was serum starved for 24h, then treated with 10% FBS for 20h whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 90-130 kDa