Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)
![Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)](https://www.abcam.com/ps/products/184/ab184797/Images/ab184797-251499-anti-rb-phospho-t252-antibody-epr17733-western-blot.jpg "Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17733] to Rb (phospho T252)
- Suitable for: Dot blot, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Rb (phospho T252) antibody [EPR17733]
See all Rb primary antibodies -
Description
Rabbit monoclonal [EPR17733] to Rb (phospho T252) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: K562 and MCF7 whole cell lysates. IHC-P: Human colon and squamous cell carcinoma of cervix tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17733 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)All lanes : Anti-Rb (phospho T252) antibody [EPR17733] (ab184797) at 1/1000 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : K562 whole cell lysate serum starved for 3 days
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 2% BSA /TBST.
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![Western blot - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)](https://www.abcam.com/ps/products/184/ab184797/Images/ab184797-251498-anti-rb-phospho-t252-antibody-epr17733-western-blot.jpg)
Western blot - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)All lanes : Anti-Rb (phospho T252) antibody [EPR17733] (ab184797) at 1/1000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : MCF7 whole cell lysate serum starved for 3 days
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 2% BSA /TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)](https://www.abcam.com/ps/products/184/ab184797/Images/ab184797-251497-anti-rb-phospho-t252-antibody-epr17733-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Rb (phospho T252) with ab184797 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nuclear staining on sporadic epithelium cells of Human colon is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)](https://www.abcam.com/ps/products/184/ab184797/Images/ab184797-251496-anti-rb-phospho-t252-antibody-epr17733-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling Rb (phospho T252) with ab184797 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nuclear staining on tumor cells of Human squamous cell carcinoma of cervix.is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Dot Blot - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)](https://www.abcam.com/ps/products/184/ab184797/Images/ab184797-251509-anti-rb-phospho-t252-antibody-epr17733-dot-blot.jpg)
Dot Blot - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)Dot blot analysis of Rb (phospho T252) phospho peptide (Lane 1) and Rb Non-phospho peptide (Lane 2) labeled using ab184797 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody (ab97051) at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)](https://www.abcam.com/ps/products/184/ab184797/Images/ab184797-7-benefits-of-recombinant-antibodies.png)
Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)
