Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17733] to Rb (phospho T252)
Suitable for: Dot blot, WB, IHC-P
Reacts with: Human

Product name

Anti-Rb (phospho T252) antibody [EPR17733]
See all Rb primary antibodies
Description

Rabbit monoclonal [EPR17733] to Rb (phospho T252)
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: K562 and MCF7 whole cell lysates. IHC-P: Human colon and squamous cell carcinoma of cervix tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17733
Isotype

IgG
Research areas

Western blot - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)

All lanes : Anti-Rb (phospho T252) antibody [EPR17733] (ab184797) at 1/1000 dilution

Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : K562 whole cell lysate serum starved for 3 days

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 106 kDa
Observed band size: 106 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 2% BSA /TBST.
Western blot - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)

All lanes : Anti-Rb (phospho T252) antibody [EPR17733] (ab184797) at 1/1000 dilution

Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : MCF7 whole cell lysate serum starved for 3 days

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 106 kDa
Observed band size: 106 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 2% BSA /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Rb (phospho T252) with ab184797 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nuclear staining on sporadic epithelium cells of Human colon is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)

Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling Rb (phospho T252) with ab184797 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nuclear staining on tumor cells of Human squamous cell carcinoma of cervix.is observed.

Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Dot Blot - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)

Dot blot analysis of Rb (phospho T252) phospho peptide (Lane 1) and Rb Non-phospho peptide (Lane 2) labeled using ab184797 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody (ab97051) at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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