Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Rabbit polyclonal to ORP-3
Suitable for: WB, IP
Reacts with: Mouse, Human
Isotype: IgG

Product name

Anti-ORP-3 antibody
See all ORP-3 primary antibodies
Description

Rabbit polyclonal to ORP-3
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide within Human ORP-3 aa 450-550. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_056365.1).
Database link: Q9H4L5

Run BLAST with Run BLAST with
Positive control
- HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.
General notes

Previously labelled as OSBPL3.

Application	Species
IP	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate

pH 7 to 8
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

ab192998 was affinity purified using an epitope specific to ORP-3 immobilized on solid support.
Clonality

Polyclonal
Isotype

IgG
Research areas

Western blot - Anti-ORP-3 antibody (ab192998)

All lanes : Anti-ORP-3 antibody (ab192998) at 0.1 µg/ml

Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate

Lysates/proteins at 50 µg per lane.

Developed using the ECL technique.

Predicted band size: 101 kDa

Exposure time: 3 minutes

Lysates prepared using NETN lysis buffer.
Immunoprecipitation - Anti-ORP-3 antibody (ab192998)

Detection of ORP-3 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab192998 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection (lane 1). Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com