Anti-LEF1 antibody (ab217378)
Key features and details
- Rabbit polyclonal to LEF1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-LEF1 antibody
See all LEF1 primary antibodies -
Description
Rabbit polyclonal to LEF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human LEF1 aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9UJU2 -
Positive control
- Mouse lymph node and human colon carcinoma lysates; rat liver tissue and human colon carcinoma tissue.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat liver tissue labeling LEF1 with ab217378 at 1/200 dilution followed by secondary detection and DAB staining.
-
All lanes : Anti-LEF1 antibody (ab217378) at 1/200 dilution
Lane 1 : rat brain lysate
Lane 2 : human colon carcinoma lysate
Secondary
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution
Predicted band size: 44 kDa
-
Anti-LEF1 antibody (ab217378) at 1/300 dilution + Mouse lymph node lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/500 dilution
Predicted band size: 44 kDa
-
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human colon carcinoma tissue labeling LEF1 with ab217378 at 1/200 dilution followed by secondary detection and DAB staining.