Anti-SOX1 antibody (ab87775)
Key features and details
- Rabbit polyclonal to SOX1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-SOX1 antibody
See all SOX1 primary antibodies -
Description
Rabbit polyclonal to SOX1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Human SOX1 aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab138973)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications RatRabbitDogHumanChimpanzeeOrangutanApplication Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).Target
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Function
Transcriptional activator. -
Tissue specificity
Mainly expressed in the developing central nervous system. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6656 Human
- Entrez Gene: 20664 Mouse
- Omim: 602148 Human
- SwissProt: O00570 Human
- SwissProt: P53783 Mouse
- Unigene: 202526 Human
- Unigene: 39088 Mouse
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Alternative names
- Sex determining region Y box 1 antibody
- sox1 antibody
- SOX1_HUMAN antibody
see all
Images
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Western blot - Anti-SOX1 antibody (ab87775)All lanes : Anti-SOX1 antibody (ab87775) at 1 µg/ml
Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 2 : 46C (Mouse embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible non-specific binding)
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% BSA before being incubated with ab87775 overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody, and visualised using ECL development solution ab133406.
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Western blot - Anti-SOX1 antibody (ab87775)This image is courtesy of an abreview by Patrick Ovando RocheAll lanes : Anti-SOX1 antibody (ab87775) at 1/1000 dilution
Lane 1 : H1 Human embryonic stem cells
Lane 2 : H1 Human embryonic stem cell derived neural progenitor stem cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Mouse anti-rabbit HRP conjugated at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
References (16)
ab87775 has been referenced in 16 publications.
- Sharma Y et al. In vitro human stem cell derived cultures to monitor calcium signaling in neuronal development and function. Wellcome Open Res 5:16 (2020). PubMed: 32195361
- Li X et al. Odontogenesis and neuronal differentiation characteristics of periodontal ligament stem cells from beagle dog. J Cell Mol Med 24:5146-5151 (2020). PubMed: 32202359
- Logun MT et al. Surfen-mediated blockade of extratumoral chondroitin sulfate glycosaminoglycans inhibits glioblastoma invasion. FASEB J 33:11973-11992 (2019). PubMed: 31398290
- Lei A et al. EZH2 Regulates Protein Stability via Recruiting USP7 to Mediate Neuronal Gene Expression in Cancer Cells. Front Genet 10:422 (2019). ICC/IF ; Mouse . PubMed: 31130994
- Shrestha R et al. Aberrant hiPSCs-Derived from Human Keratinocytes Differentiates into 3D Retinal Organoids that Acquire Mature Photoreceptors. Cells 8:N/A (2019). PubMed: 30634512
Images
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Western blot - Anti-SOX1 antibody (ab87775)All lanes : Anti-SOX1 antibody (ab87775) at 1 µg/ml
Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 2 : 46C (Mouse embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible non-specific binding)
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% BSA before being incubated with ab87775 overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody, and visualised using ECL development solution ab133406.
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Western blot - Anti-SOX1 antibody (ab87775) This image is courtesy of an abreview by Patrick Ovando RocheAll lanes : Anti-SOX1 antibody (ab87775) at 1/1000 dilution
Lane 1 : H1 Human embryonic stem cells
Lane 2 : H1 Human embryonic stem cell derived neural progenitor stem cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Mouse anti-rabbit HRP conjugated at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
