Anti-IGFBP1 antibody (ab228741)
Key features and details
- Rabbit polyclonal to IGFBP1
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IGFBP1 antibody
See all IGFBP1 primary antibodies -
Description
Rabbit polyclonal to IGFBP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human IGFBP1 (internal sequence). The exact sequence is proprietary.
Database link: P08833 -
Positive control
- WB: HepG2 whole cell extract. IP: HepG2 whole cell extract. ICC/IF: HepG2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IGFBP1 antibody (ab228741)Anti-IGFBP1 antibody (ab228741) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg
Predicted band size: 28 kDa12% SDS-PAGE gel.
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Immunoprecipitation - Anti-IGFBP1 antibody (ab228741)IGFBP1 was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract with 5 µg of ab228741. Western blot was performed from the immunoprecipitate using ab228741.
Lane 1: HepG2 whole cell extract (Input).
Lane 2: Control IgG instead of ab228741 in HepG2 whole cell extract.
Lane 3: ab228741 IP in HepG2 whole cell extract. -
Immunocytochemistry/ Immunofluorescence - Anti-IGFBP1 antibody (ab228741)HepG2 cells stained for IGFBP1 (green) using ab228741 at 1/500 dilution in ICC/IF.
