Anti-NUCB2 antibody (ab224348)
Key features and details
- Rabbit polyclonal to NUCB2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NUCB2 antibody
See all NUCB2 primary antibodies -
Description
Rabbit polyclonal to NUCB2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human NUCB2 aa 300 to the C-terminus.
Database link: P80303 -
Positive control
- WB: HL-60 and NBT-II cell lysate. IHC-P: Human stomach tissue. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUCB2 antibody (ab224348)
Paraffin-embedded human stomach tissue stained for NUCB2 using ab224348 at 1/1000 in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-NUCB2 antibody (ab224348)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for NUCB2 (green) using ab224348 at 4 µg/ml in ICC/IF.
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Western blot - Anti-NUCB2 antibody (ab224348)All lanes : Anti-NUCB2 antibody (ab224348) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Developed using the ECL technique.
Predicted band size: 50 kDa
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Western blot - Anti-NUCB2 antibody (ab224348)Anti-NUCB2 antibody (ab224348) at 1/100 dilution + HL-60 (human promyelocytic leukemia cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 50 kDa
