Anti-NUCB2 antibody - C-terminal (ab229683)
Key features and details
- Rabbit polyclonal to NUCB2 - C-terminal
- Suitable for: IHC-P, WB, ICC/IF, IP
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-NUCB2 antibody - C-terminal
See all NUCB2 primary antibodies -
Description
Rabbit polyclonal to NUCB2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment within Human NUCB2 (C terminal). The exact sequence is proprietary.
Database link: P80303 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract. IHC-P: Rat brain tissue. ICC/IF: A431 cells.
-
General notes
Previously labelled as Nucleobindin 2.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUCB2 antibody - C-terminal (ab229683)
Paraffin-embedded rat brain tissue stained for NUCB2 using ab229683 at 1/500 dilution in immunohistochemical analysis.
-
Western blot - Anti-NUCB2 antibody - C-terminal (ab229683)All lanes : Anti-NUCB2 antibody - C-terminal (ab229683) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 50 kDa10% SDS-PAGE gel.
-
Immunocytochemistry/ Immunofluorescence - Anti-NUCB2 antibody - C-terminal (ab229683)4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for NUCB2 (green) using ab229683 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
-
Immunoprecipitation - Anti-NUCB2 antibody - C-terminal (ab229683)NUCB2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab229683. Western blot was performed from the immunoprecipitate using ab229683. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HEK-293T whole cell extract (Input).
Lane 2: Control IgG instead of ab229683 in HEK-293T whole cell extract.
Lane 3: ab229683 IP in HEK-293T whole cell extract.
