Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Neuroscience Neurology process Notch Pathway

Anti-Notch1 antibody [N253/32] (ab167441)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Notch1 antibody [N253/32] (ab167441)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [N253/32] to Notch1
  • Suitable for: WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG1

You may also be interested in

Product image
Human NOTCH2 knockout HeLa cell lysate (ab258538)
Product image
Anti-Jagged 2/JAG2 antibody (ab10556)
Product image
Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)
Product image
Alexa Fluor® 555 Anti-GSK3 beta antibody [Y174] (ab201738)

Overview

  • Product name

    Anti-Notch1 antibody [N253/32]
    See all Notch1 primary antibodies
  • Description

    Mouse monoclonal [N253/32] to Notch1
  • Host species

    Mouse
  • Specificity

    ab167441 detects >270kDa, ~120kDa and additional small fragments due to proteolysis. ab167441 does not cross-react with Notch2 or Notch3.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Fusion protein corresponding to amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of Mouse Notch1 (UniProt Q01705).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Rat brain membrane lysate.
  • General notes

    The clone number has been updated from S253-32 to N253/32, both clone numbers name the same antibody clone.

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    N253/32
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurology process
    • Notch Pathway
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Surface Molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators
    • Developmental Biology
    • Embryogenesis
    • Somatogenesis

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Notch1 antibody [N253/32] (ab167441)
    Immunocytochemistry/ Immunofluorescence - Anti-Notch1 antibody [N253/32] (ab167441)

    Immunocytochemistry/Immunofluorescence analysis using ab167441 at 1/100 in Neuroblastoma cell line (SK-N-BE). Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody incubation for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain 1/1000 for 60 min at RT; DAPI (blue) nuclear stain at 1/5000 for 5min RT. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab167441 (D) Composite.

     

  • Western blot - Anti-Notch1 antibody [N253/32] (ab167441)
    Western blot - Anti-Notch1 antibody [N253/32] (ab167441)
    Anti-Notch1 antibody [N253/32] (ab167441) at 1/1000 dilution + Rat Brain membrane lysate. at 10 µg

    Secondary
    HRP conjugated Goat Anti-Mouse IgG at 1/200 dilution

    Predicted band size: 271 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Notch1 antibody [N253/32] (ab167441)

  •  
  • Product image

    Alexa Fluor® 647 Anti-Notch1 antibody [EP1238Y] (ab194122)

    Applications: ICC/IF

  •  
  • Product image

    Anti-Notch1 antibody [EP1238Y] - Low endotoxin, Azide free (ab246693)

    Applications: Flow Cyt, ICC/IF, IHC-P, WB

  •  
  • Product image

    HRP Anti-Notch1 antibody [EP1238Y] (ab194123)

    Applications: WB

  •  
  • Product image

    Anti-Notch1 antibody [EP1238Y] (ab52627)

    Applications: Flow Cyt, ICC/IF, IHC-P, WB

  •  
  • Product image

    Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (ab221603)

    Applications: Flow Cyt, ICC/IF, IHC-P, WB

  •  
  • Product image

    Anti-Notch1 antibody (ab65297)

    Applications: IP, WB

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.