Anti-Notch1 antibody [N253/32] (ab167441)
Key features and details
- Mouse monoclonal [N253/32] to Notch1
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Notch1 antibody [N253/32]
See all Notch1 primary antibodies -
Description
Mouse monoclonal [N253/32] to Notch1 -
Host species
Mouse -
Specificity
ab167441 detects >270kDa, ~120kDa and additional small fragments due to proteolysis. ab167441 does not cross-react with Notch2 or Notch3. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Fusion protein corresponding to amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of Mouse Notch1 (UniProt Q01705).
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Positive control
- Rat brain membrane lysate.
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General notes
The clone number has been updated from S253-32 to N253/32, both clone numbers name the same antibody clone.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N253/32 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/Immunofluorescence analysis using ab167441 at 1/100 in Neuroblastoma cell line (SK-N-BE). Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody incubation for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain 1/1000 for 60 min at RT; DAPI (blue) nuclear stain at 1/5000 for 5min RT. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab167441 (D) Composite.
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Anti-Notch1 antibody [N253/32] (ab167441) at 1/1000 dilution + Rat Brain membrane lysate. at 10 µg
Secondary
HRP conjugated Goat Anti-Mouse IgG at 1/200 dilution
Predicted band size: 271 kDa