Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Eph receptor B3 antibody [EPR8280] (ab133742)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8280] to Eph receptor B3
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Eph receptor B3 antibody [EPR8280]
    See all Eph receptor B3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8280] to Eph receptor B3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Eph receptor B3 aa 100-200 (extracellular). The exact sequence is proprietary.

  • Positive control

    • WB: Jurkat, HepG2, HeLa, MOLT4, NIH3T3, Rat-1 and C6 cell lysates. Rat spinal cord tissue lysate; ICC/IF: Jurkat and HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Eph receptor B3 antibody [EPR8280] (ab133742)
    Western blot - Anti-Eph receptor B3 antibody [EPR8280] (ab133742)
    All lanes : Anti-Eph receptor B3 antibody [EPR8280] (ab133742) at 1/1000 dilution (Purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
    Lane 2 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
    Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysates
    Lane 7 : Rat spinal cord lysates
    Lane 8 : Rat-1 (Rat embryonic fibroblast) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 110 kDa
    Observed band size: 110 kDa



    Blocking/Diluting buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] (ab133742)
    Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] (ab133742)
    Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Eph receptor B3 with Purified ab133742 at 1/50 dilution (5.14 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Western blot - Anti-Eph receptor B3 antibody [EPR8280] (ab133742)
    Western blot - Anti-Eph receptor B3 antibody [EPR8280] (ab133742)
    All lanes : Anti-Eph receptor B3 antibody [EPR8280] (ab133742) at 1/1000 dilution (unpurified)

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : MOLT4 cell lysate
    Lane 5 : NIH3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 110 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] (ab133742)
    Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] (ab133742)

    Immunocytochemical analysis of HeLa cells labelling Eph receptor B3 with unpurified ab133742 at 1/100.

  • OI-RD Scanning - Anti-Eph receptor B3 antibody [EPR8280] (ab133742)
    OI-RD Scanning - Anti-Eph receptor B3 antibody [EPR8280] (ab133742)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Eph receptor B3 antibody [EPR8280] (ab133742)
    Anti-Eph receptor B3 antibody [EPR8280] (ab133742)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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