Anti-Myelin PLP antibody (ab105784)
Key features and details
- Rabbit polyclonal to Myelin PLP
- Suitable for: IHC-Fr, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Myelin PLP antibody
See all Myelin PLP primary antibodies -
Description
Rabbit polyclonal to Myelin PLP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Rabbit, Goat, Chicken, Cow, Cat, Dog, Human, Pig, Xenopus laevis, Common marmoset, Orangutan
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Immunogen
Synthetic peptide corresponding to Rat Myelin PLP aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab124616) -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab105784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-Fr Use at an assay dependent concentration. WB Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 30 kDa). Target
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Function
This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin. -
Involvement in disease
Defects in PLP1 are the cause of leukodystrophy hypomyelinating type 1 (HLD1) [MIM:312080]; also known as Pelizaeus-Merzbacher disease. HLD1 is an X-linked recessive dysmyelinating disorder of the central nervous system in which myelin is not formed properly. It is characterized clinically by nystagmus, spastic quadriplegia, ataxia, and developmental delay.
Defects in PLP1 are the cause of spastic paraplegia X-linked type 2 (SPG2) [MIM:312920]. SPG2 is characterized by spastic gait and hyperreflexia. In some patients, complicating features include nystagmus, dysarthria, sensory disturbance, mental retardation, optic atrophy. -
Sequence similarities
Belongs to the myelin proteolipid protein family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 281410 Cow
- Entrez Gene: 481002 Dog
- Entrez Gene: 5354 Human
- Entrez Gene: 18823 Mouse
- Entrez Gene: 24943 Rat
- Omim: 300401 Human
- SwissProt: P04116 Cow
- SwissProt: P23294 Dog
see all -
Alternative names
- HLD1 antibody
- Lipophilin antibody
- Major myelin proteolipid protein antibody
see all
Images
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Western blot - Anti-Myelin PLP antibody (ab105784)All lanes : Anti-Myelin PLP antibody (ab105784) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute -
Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody (ab105784)Image courtesy of Carl Hobbs (Imperial College London, United Kingdom)IHC-Fr image of myelin PLP staining on Mouse sciatic nerve sections using ab105784 (1:3000). Fresh frozen sections (10μM) and dried in fumehood for 2 hours before fixing with 10% formalin for 10 min. Sections were permeablized in 0.1% Triton X-100 in TBS for 10min. Sections were then blocked using 1% BSA for 10 mins at 21°C. ab105784 was diluted 1:2000 in TBS buffer (containing BSA) and sections were then incubated with ab105784 for 16 hours at 21°C. The secondary antibody used was Alexa Fluor® 594 conjugated Goat polyclonal to Rabbit IgG (1:1000).
Protocols
References (7)
ab105784 has been referenced in 7 publications.
- Zhou X et al. Mature myelin maintenance requires Qki to coactivate PPARß-RXRa-mediated lipid metabolism. J Clin Invest 130:2220-2236 (2020). PubMed: 32202512
- Goncalves MB et al. Regulation of Myelination by Exosome Associated Retinoic Acid Release from NG2-Positive Cells. J Neurosci 39:3013-3027 (2019). PubMed: 30760627
- Sun LO et al. Spatiotemporal Control of CNS Myelination by Oligodendrocyte Programmed Cell Death through the TFEB-PUMA Axis. Cell 175:1811-1826.e21 (2018). PubMed: 30503207
- Dheer A et al. Temporal and Spatial Changes in Glial Cells During Chronic Hypobaric Hypoxia: Role in Neurodegeneration. Neuroscience 383:235-246 (2018). PubMed: 29751055
- Montagne A et al. Pericyte degeneration causes white matter dysfunction in the mouse central nervous system. Nat Med 24:326-337 (2018). PubMed: 29400711
- van Tilborg E et al. A quantitative method for microstructural analysis of myelinated axons in the injured rodent brain. Sci Rep 7:16492 (2017). PubMed: 29184182
- Wlodarczyk A et al. A novel microglial subset plays a key role in myelinogenesis in developing brain. EMBO J 36:3292-3308 (2017). IHC-Fr ; Mouse . PubMed: 28963396
Images
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Western blot - Anti-Myelin PLP antibody (ab105784)All lanes : Anti-Myelin PLP antibody (ab105784) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
-
Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody (ab105784) Image courtesy of Carl Hobbs (Imperial College London, United Kingdom)
IHC-Fr image of myelin PLP staining on Mouse sciatic nerve sections using ab105784 (1:3000). Fresh frozen sections (10μM) and dried in fumehood for 2 hours before fixing with 10% formalin for 10 min. Sections were permeablized in 0.1% Triton X-100 in TBS for 10min. Sections were then blocked using 1% BSA for 10 mins at 21°C. ab105784 was diluted 1:2000 in TBS buffer (containing BSA) and sections were then incubated with ab105784 for 16 hours at 21°C. The secondary antibody used was Alexa Fluor® 594 conjugated Goat polyclonal to Rabbit IgG (1:1000).
