Anti-NNT antibody [8B4BB10] (ab110352)
![Anti-NNT antibody [8B4BB10] (ab110352)](https://www.abcam.com/ps/products/110/ab110352/Images/ab110352-123663-anti-nnt-antibody-8b4bb10-western-blot.jpg "Anti-NNT antibody [8B4BB10] (ab110352)")
Key features and details
- Mouse monoclonal [8B4BB10] to NNT
- Suitable for: IP, ICC/IF, IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
Overview
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Product name
Anti-NNT antibody [8B4BB10]
See all NNT primary antibodies -
Description
Mouse monoclonal [8B4BB10] to NNT -
Host species
Mouse -
Specificity
Several customers have found that this antibody may not work well for mouse and rat samples.
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Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanIP MouseRatCowHumanWB Human
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Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- Normal Human skin fibroblasts (strain HDFn); Human cerebellum tissue; mitochondria from Human liver, Bovine heart, Rat liver and Mouse liver; HepG2 and HL-60 cells.
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
8B4BB10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-NNT antibody [8B4BB10] (ab110352)Anti-NNT antibody [8B4BB10] (ab110352) at 0.5 µg/ml + HepG2 whole cell lysate
Predicted band size: 114 kDa
anti-Complex II 30kDa subunit has also been labeled as a control. -
![Flow Cytometry - Anti-NNT antibody [8B4BB10] (ab110352)](https://www.abcam.com/ps/products/110/ab110352/Images/ab110352-123667-anti-nnt-antibody-8b4bb10-flow-cytometry.jpg)
Flow Cytometry - Anti-NNT antibody [8B4BB10] (ab110352)HL-60 cells were stained with 1 µg/mL NNT antibody (ab110352) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry. -
![Immunocytochemistry/ Immunofluorescence - Anti-NNT antibody [8B4BB10] (ab110352)](https://www.abcam.com/ps/products/110/ab110352/Images/ab110352-123666-anti-nnt-antibody-8b4bb10-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NNT antibody [8B4BB10] (ab110352)Mitochondrial localization of NNT in cultured, normal Human skin fibroblasts (strain HDFn) labeled with anti NNT (ab110352) as the primary antibody and an AlexaFluor® 594 goat anti-mouse IgG1 secondary antibody. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNT antibody [8B4BB10] (ab110352)](https://www.abcam.com/ps/products/110/ab110352/Images/ab110352-123665-anti-nnt-antibody-8b4bb10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNT antibody [8B4BB10] (ab110352)NNT immunohistochemistry in Human cerebellum visualized with ab110352 at 1/100. NNT immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells. -
![Immunoprecipitation - Anti-NNT antibody [8B4BB10] (ab110352)](https://www.abcam.com/ps/products/110/ab110352/Images/ab110352-123664-anti-nnt-antibody-8b4bb10-immunoprecipitation.jpg)
Immunoprecipitation - Anti-NNT antibody [8B4BB10] (ab110352)Nicotinamide nucleotide transhydrogenase (NNT) immunocaptured from Human liver mitochondria (lane 1), bovine heart mitochondria (lane 2), rat liver mitochondria (lane 3), mouse liver mitochondria (lane 4), and HepG2 cells (lane 5), using ab110352.