Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NDUFB7 antibody [EPR15903] (ab188575)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15903] to NDUFB7
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-NDUFB7 antibody [EPR15903]
    See all NDUFB7 primary antibodies

  • Description

    Rabbit monoclonal [EPR15903] to NDUFB7

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • A431, HL60 and PC3 cell lysates. Human papillary adenocarcinoma of thyroid tissue. PC3 and HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NDUFB7 antibody [EPR15903] (ab188575)
    Western blot - Anti-NDUFB7 antibody [EPR15903] (ab188575)
    All lanes : Anti-NDUFB7 antibody [EPR15903] (ab188575) at 1/1000 dilution

    Lane 1 : PC3 cell lysate
    Lane 2 : HL60 cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 16 kDa
    Observed band size: 16 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB7 antibody [EPR15903] (ab188575)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB7 antibody [EPR15903] (ab188575)

    Immunohistochemical analysis of paraffin-embedded, Human papillary adenocarcinoma of thyroid tissue labelling NDUFB7 with ab188575 at a 1/100 dilution. Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NDUFB7 antibody [EPR15903] (ab188575)
    Immunocytochemistry/ Immunofluorescence - Anti-NDUFB7 antibody [EPR15903] (ab188575)

    Immunofluorescence analysis of, 4% paraformaldehyde-fixed, HeLa cells labelling NDUFB7 with ab188575 at a 1/250 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.

  • Flow Cytometry - Anti-NDUFB7 antibody [EPR15903] (ab188575)
    Flow Cytometry - Anti-NDUFB7 antibody [EPR15903] (ab188575)

    Flow cytometry analysis of 2% paraformaldehyde-fixed PC3 cells labelling NDUFB7 with ab188575 at a 1/340 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).

  • Immunoprecipitation - Anti-NDUFB7 antibody [EPR15903] (ab188575)
    Immunoprecipitation - Anti-NDUFB7 antibody [EPR15903] (ab188575)

    Western blot analysis on immunoprecipitation pellet from A431 cell lysate labelling NDUFB7 with ab188575 at 1/50 dilution and goat anti-rabbit IgG, (H+L), peroxidase conjugated at a 1/1000 dilution.

     

  • Anti-NDUFB7 antibody [EPR15903] (ab188575)
    Anti-NDUFB7 antibody [EPR15903] (ab188575)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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