Anti-NMNAT2 antibody (ab110040)
Key features and details
- Rabbit polyclonal to NMNAT2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NMNAT2 antibody
See all NMNAT2 primary antibodies -
Description
Rabbit polyclonal to NMNAT2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chinese hamster
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: This antibody gave a positive signal in the following tissue lysates: Rat Brain; Mouse Brain; Rat Cortex; Mouse Cortex; Rat Forebrain. ICC/IF: This antibody gave a positive result in IF in the following Methanol fixed cell line: SKNSH
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab110040 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa). ICC/IF Use a concentration of 5 µg/ml. Target
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Function
Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency. Cannnot use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity prefers NAD(+), NADH and NAAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). -
Tissue specificity
Highly expressed in brain, in particular in cerebrum, cerebellum, occipital lobe, frontal lobe, temporal lobe and putamen. Also found in the heart, skeletal muscle, pancreas and islets of Langerhans. -
Pathway
Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1. -
Sequence similarities
Belongs to the eukaryotic NMN adenylyltransferase family. -
Cellular localization
Cytoplasm. Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 23057 Human
- Entrez Gene: 226518 Mouse
- Entrez Gene: 289095 Rat
- Omim: 608701 Human
- SwissProt: Q9BZQ4 Human
- SwissProt: Q8BNJ3 Mouse
- SwissProt: Q0HA29 Rat
- Unigene: 497123 Human
see all -
Alternative names
- C1orf15 antibody
- KIAA0479 antibody
- MGC2756 antibody
see all
Images
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Western blot - Anti-NMNAT2 antibody (ab110040)All lanes : Anti-NMNAT2 antibody (ab110040) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Rat Cortex Tissue Lysate
Lane 4 : Mouse Cortex Tissue Lysate
Lane 5 : Rat Forebrain Rat Tissue Lysate
Lane 6 : Lung (Rat) Tissue Lysate
Lane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Both Rat Lung tissue lysate and NIH3T3 whole cell lysate were used as negative controls for Nicotinamide mononucleotide adenylyltransferase 2 (NMNAT2) -
Immunocytochemistry/ Immunofluorescence - Anti-NMNAT2 antibody (ab110040)ab110040 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab110040 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (1)
ab110040 has been referenced in 1 publication.
- Ali YO et al. Screening with an NMNAT2-MSD platform identifies small molecules that modulate NMNAT2 levels in cortical neurons. Sci Rep 7:43846 (2017). PubMed: 28266613
Images
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Western blot - Anti-NMNAT2 antibody (ab110040)All lanes : Anti-NMNAT2 antibody (ab110040) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Rat Cortex Tissue Lysate
Lane 4 : Mouse Cortex Tissue Lysate
Lane 5 : Rat Forebrain Rat Tissue Lysate
Lane 6 : Lung (Rat) Tissue Lysate
Lane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Both Rat Lung tissue lysate and NIH3T3 whole cell lysate were used as negative controls for Nicotinamide mononucleotide adenylyltransferase 2 (NMNAT2) -
Immunocytochemistry/ Immunofluorescence - Anti-NMNAT2 antibody (ab110040)ab110040 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab110040 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
