Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20328] to nkx6.1 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free
    See all nkx6.1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20328] to nkx6.1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251565 is the carrier-free version of ab221544. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251565 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)

    This data was developed using ab221544, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling nkx6.1 with ab221544 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on islet of human pancreas (PMID: 8702531). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)

    This data was developed using ab221544, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human pancreatic neuroendocrine neoplasm tissue labeling nkx6.1 with ab221544 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Mainly nuclear staining on tumor cells of human pancreatic neuroendocrine neoplasm (PMID: 25871618). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    This data was developed using ab221544, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling nkx6.1 with ab221544 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on islet of mouse pancreas (PMID: 8702531). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    This data was developed using ab221544, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling nkx6.1 with ab221544 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on rat pancreas (PMID: 8702531). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    This data was developed using ab221544, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human colon tissue labeling nkx6.1 with ab221544 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Negative control: no staining on human colon (PMID: 25871618). Counter stained with hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    Western blot - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    Anti-nkx6.1 antibody [EPR20328] (ab221544) at 1/1000 dilution + Human nkx6.1 recombinant protein (aa (1-48) & aa(66-120) & aa(179-240) with a His-tag) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 38 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    This data was developed using ab221544, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Human nkx6.1 recombinant protein contains aa1-48, aa66-120, and aa179-240 with a His-tag.

  • Western blot - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    Western blot - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    Anti-nkx6.1 antibody [EPR20328] (ab221544) at 1/200 dilution + Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa


    Exposure time: 3 minutes


    This data was developed using ab221544, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)

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