Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free (ab250875)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14970] to Nkx3.1 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free
    See all Nkx3.1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14970] to Nkx3.1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250875 is the carrier-free version of ab186413. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250875 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free (ab250875)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free (ab250875)
    This data was developed using ab186413, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded human prostate tissue labeling Nkx3.1 with ab186413 at 1/4000 dilution and ready to use HRP Polymer to Rabbit IgG. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Western blot - Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free (ab250875)
    Western blot - Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free (ab250875)
    All lanes : Anti-Nkx3.1 antibody [EPR14970] (ab186413) at 0.988 µg/ml (Exposure time: 10 seconds)

    Lane 1 : LNCaP cell lysate
    Lane 2 : PC3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 26 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab186413, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free (ab250875)
    Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free (ab250875)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free (ab250875)

© 2021 Astana Biomed Group LLP. All rights reserved.