Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free (ab250875)
![Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free (ab250875)](https://www.abcam.com/ps/products/250/ab250875/Images/ab250875-2-anti-nkx31-antibody-epr14970-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg "Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free (ab250875)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14970] to Nkx3.1 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free
See all Nkx3.1 primary antibodies -
Description
Rabbit monoclonal [EPR14970] to Nkx3.1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250875 is the carrier-free version of ab186413. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250875 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR14970 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free (ab250875)This data was developed using ab186413, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded human prostate tissue labeling Nkx3.1 with ab186413 at 1/4000 dilution and ready to use HRP Polymer to Rabbit IgG. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free (ab250875)](https://www.abcam.com/ps/products/250/ab250875/Images/ab250875-403226-anti-nkx31-antibody-epr14970-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free (ab250875)All lanes : Anti-Nkx3.1 antibody [EPR14970] (ab186413) at 0.988 µg/ml (Exposure time: 10 seconds)
Lane 1 : LNCaP cell lysate
Lane 2 : PC3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?This data was developed using ab186413, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free (ab250875)](https://www.abcam.com/ps/products/250/ab250875/Images/ab250875-3-benefits-of-recombinant-antibodies.png)
Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free (ab250875)
