Anti-NIPA antibody (ab176847)
Key features and details
- Rabbit polyclonal to NIPA
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NIPA antibody
See all NIPA primary antibodies -
Description
Rabbit polyclonal to NIPA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human NIPA aa 300-350. The exact sequence is proprietary. NP_057562.3
Sequence:LTSSPIPGLEG RPERLPLVPE SPRRMMTRSQ DATFSPGSEQ AEKSPGPIVS
Database link: Q86WB0 -
Positive control
- HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab176847 was affinity purified using an epitope specific to NIPA immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NIPA antibody (ab176847)All lanes : Anti-NIPA antibody (ab176847) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 55 kDa
Exposure time: 10 seconds
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Immunoprecipitation - Anti-NIPA antibody (ab176847)Detection of NIPA in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176847 at 3 µg/mg lysate for IP (Lane 1). For WB detection ab176847 was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
