Recombinant human KDM6A / UTX protein (Active) (ab271785)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 58% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human KDM6A / UTX protein (Active)
See all KDM6A / UTX proteins and peptides -
Biological activity
Specific Activity: 0.096 pmol/min/μg.Assay Conditions: 10 µl reaction mix containing assay buffer with 50 mM HEPES (pH 7.4), 1 mM TCEP, 100 µM alpha-ketoglutarate, 25 µM iron, 0.1 mM ascorbic acid, 0.02% Tween-20, 0.5 µM biotinylated peptide substrate, and ab271785 (10 – 50ng) added to the wells. Add antibody against di-methylated K27 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection. -
Purity
>= 58 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALG GLDSRLFGFV RFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQ LGHFNLLLEDYPKALSAYQRYY SLQSDYWKNAAFLYGLGLVYFHYNAF QWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVN TDYESSLKHFQLAL VDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQ VK ATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGR CYSSIGKV QDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQA YICAVQLDHGHAAAWMDLGT LYESCNQPQDAIKCYLNATRSKSCSNTS ALAARIKYLQAQLCNLPQGSLQNKTKLLPSIE EAWSLPIPAELTSRQG AMNTAQQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQL RANR NNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLP TNSVSG QQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTS RTLGSTDTILIGNNHITG SGSNGNVPYLQRNALTLPHNRTNLTSSAEE PWKNQLSNSTQGLHKGQSSHSAGPNGERPL SSTGPSQHLQAAGSGIQN QNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKP SGNILT VPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSD NPQL SALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAIS TATPSPKSTEQTTTNS VTSLNSPHSGLHTINGEGMEESQSPMKTDLLL VNHKPSPQIIPSMSVSIYPSSAEVLKAC RNLGKNGLSNSSILLDKCPP PRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFC TNPNNPVT VIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTK KI WHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSEST SSDNSGRRRKGPFK TIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVS AGNLLSHVGHTILGMNTVQLYMKVPG SRTPGHQENNNFCSVNINIGPG DCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDL YEANVPVYRF IQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNK LQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLRE ALIAAGKEIIWH GRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIV HCQDCARKTSGNLENFVVLEQYKM EDLMQVYDQFTLAPPLPSASS -
Molecular weight information
155 by SDS-PAGE -
Amino acids
1 to 1401 -
Tags
DDDDK tag N-Terminus
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Preparation and Storage
- bA386N14.2
- bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX)
- bA386N14.2 ubiquitously transcribed X chromosome tetratricopeptide repeat protein UTX
Images
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Functional Studies - Recombinant human KDM6A / UTX protein (Active) (ab271785)
Specific activity of ab271785 was 0.096 pmol/min/μg.
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SDS-PAGE - Recombinant human KDM6A / UTX protein (Active) (ab271785)SDS-PAGE analysis of 1.2 µg ab271785.