Anti-NIPA (phospho S354) antibody (ab63557)
Key features and details
- Rabbit polyclonal to NIPA (phospho S354)
- Suitable for: WB
- Reacts with: African green monkey
- Isotype: IgG
Overview
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Product name
Anti-NIPA (phospho S354) antibody
See all NIPA primary antibodies -
Description
Rabbit polyclonal to NIPA (phospho S354) -
Host species
Rabbit -
Specificity
Detects NIPA only when phosphorylated at serine 354. -
Tested Applications & Species
See all applications and species dataApplication Species WB African green monkey
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Immunogen
Synthetic phosphopeptide derived from human NIPA around the phosphorylation site of serine 354 (T-R-SP-W-D).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NIPA (phospho S354) antibody (ab63557)All lanes : Anti-NIPA (phospho S354) antibody (ab63557) at 1/500 dilution
Lane 1 : extracts from COS7 cells, treated
with HU (2nM, 24hours)
Lane 2 : extracts from COS7 cells, treated
with HU (2nM, 24hours) with immunizing phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Additional bands at: 30 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
