Anti-Ninein/NIN antibody - Centrosome Marker (ab4447)
Key features and details
- Rabbit polyclonal to Ninein/NIN - Centrosome Marker
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Ninein/NIN antibody - Centrosome Marker
See all Ninein/NIN primary antibodies -
Description
Rabbit polyclonal to Ninein/NIN - Centrosome Marker -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
-
Immunogen
Fusion protein corresponding to Human Ninein/NIN. The region used shows good homology to Mouse Ninein/NIN aa 1-550.
-
General notes
The centrosomal protein ninein has been identified as a microtubule minus end capping and centriole anchoring protein, but its true physiological function remains to be determined. At mitosis, ninein has been observed in association with the mitotic spindle as well as at the centrosomes.
This product was previously labelled as Ninein.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
The centrosomal protein ninein has been identified as a microtubule minus end capping and centriole anchoring protein, but its true physiological function remains to be determined. At mitosis, ninein has been observed in association with the mitotic spindle as well as at the centrosomes. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Ninein/NIN antibody - Centrosome Marker (ab4447) Gordon Chan, University of Alberta
IF using ab4447 to detect Ninein/NIN in MCF7 cells (human).
The left hand panels are DAPI stained nuclei. The right hand panels are the same nuclei stained for Ninein/NIN.
ab4447 was used at 1/1000.
Ninein/NIN shows nuclear and centrosome staining, which is expected from the literature.
-
Immunocytochemistry/ Immunofluorescence - Anti-Ninein/NIN antibody - Centrosome Marker (ab4447)ICC/IF image of ab4447 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab4447, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
ab4447 shows the characteristic nuclear and centrosome staining for Ninein/NIN.
